AB1927

Mouse monoclonal [2C11] Anti-Human IgG1 Fc

Name: Mouse Anti-Human IgG1 Fc secondary antibody (ab1927) | Abcam
Brand: Abcam Limited
SKU: AB1927
Rating: 5 (1 reviews)

(1 Review)

(14 Publications)

Mouse Anti-Human IgG1 Fc (ab1927) is an unconjugated secondary antibody that is suitable for IHC and ELISA applications.

- Suitable for custom labeling or when non-standard detection methods are required
- Cited in over 10 publications

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

1 Images

ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (AB1927)

ELISA

PubMed

ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (AB1927)

Characterisation of virus activity and functional expression of monoclonal antibodies.

(Panel B) IgG1 antibody expression detected in cellular supernatants of HT-29 cells infected with 10ppc NG-135 by anti-IgG1 ELISA.

Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates (NUNC) were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (ab1927, Abcam) at a 1 : 1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.

Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Unconjugated

Anti-PIP2 antibody [2C11]

Key facts

Host species

Mouse

Target species

Human

Target isotype

IgG1

Target specificity

Fc region

Minimal cross-reactivity

Pre-adsorbed

Applications

ELISA, IHC-Fr

applications

Clonality

Monoclonal

Clone number

2C11

Isotype

IgG1

Immunogen

Native Full Length Protein corresponding to Human IGHG1.

P01857

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Chromatography on protein A Sepharose

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

See full target information IGHG1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 109:3167-3181 PubMed38813729

2024

Single-cell CRISPR screening characterizes transcriptional deregulation in T-cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Meyers,Olga Gielen,Jan Cools,Sofie Demeyer

Development (Cambridge, England) 150: PubMed37260147

2023

RTK signalling promotes epithelial columnar cell shape and apical junction maintenance in human lung progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyu Liu,Dawei Sun,Richard Butler,Emma L Rawlins

Nature communications 12:4164 PubMed34230493

2021

Oncogenic cooperation between TCF7-SPI1 and NRAS(G12D) requires β-catenin activity to drive T-cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Quentin Van Thillo,Jolien De Bie,Janith A Seneviratne,Sofie Demeyer,Sofia Omari,Anushree Balachandran,Vicki Zhai,Wai L Tam,Bram Sweron,Ellen Geerdens,Olga Gielen,Sarah Provost,Heidi Segers,Nancy Boeckx,Glenn M Marshall,Belamy B Cheung,Kiyotaka Isobe,Itaru Kato,Junko Takita,Timothy G Amos,Ira W Deveson,Hannah McCalmont,Richard B Lock,Ethan P Oxley,Maximilian M Garwood,Ross A Dickins,Anne Uyttebroeck,Daniel R Carter,Jan Cools,Charles E de Bock

Respiratory research 20:194 PubMed31443651

2019

Inhibition of lung cancer cells and Ras/Raf/MEK/ERK signal transduction by ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7).

Applications

Unspecified application

Species

Unspecified reactive species

Zhongwei Wen,Rongfang Jiang,Ying Huang,Zhineng Wen,Dong Rui,Xiaoxiao Liao,Zhougui Ling

Medical science monitor : international medical jo 25:5747-5756 PubMed31374070

2019

Upregulation of Insulin-Like Growth Factor-1 Receptor (IGF-1R) Reverses the Inhibitory Effect of Let-7g-5p on Migration and Invasion of Nasopharyngeal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhecheng Zhao,Jianxue Wen,Lihua Peng,Hanbo Liu

Cancer biology & therapy 20:1223-1233 PubMed31132019

2019

Hematopoietic-substrate-1 associated protein X-1 (HAX-1) regulates liver cancer cells growth, metastasis, and angiogenesis through Akt.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Wu,Xiangnan Ai,Hao Hu,Siqi Wang,Yang Wang,Feng Kang,Caiguo Ouyang,Jiye Zhu

Neoplasma 66:584-592 PubMed31058534

2019

Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Y Gao,H Zhu,Q Mao

Cell cycle (Georgetown, Tex.) 18:425-436 PubMed30700227

2019

Sirtuin 6 overexpression relieves sepsis-induced acute kidney injury by promoting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Ling Wang,Lei Meng,Guangke Cao,Yu Wu

Cancer biology & therapy 20:680-691 PubMed30590988

2018

Krüppel like factor 6 splice variant 1 (KLF6-SV1) overexpression recruits macrophages to participate in lung cancer metastasis by up-regulating TWIST1.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Xiao Wang,Yawei Wang,Shuguang Li,Xiuwen Wang

Cancer cell 34:271-285.e7 PubMed30107177

2018

Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Marlies Vanden Bempt,Sofie Demeyer,Michaël Broux,Jolien De Bie,Simon Bornschein,Nicole Mentens,Roel Vandepoel,Ellen Geerdens,Enrico Radaelli,Beat C Bornhauser,Andreas E Kulozik,Jules P Meijerink,Jean-Pierre Bourquin,Charles E de Bock,Jan Cools

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse monoclonal [2C11] Anti-Human IgG1 Fc

ELISA - Mouse monoclonal [2C11] Anti-Human IgG1 Fc (AB1927)

Related conjugates and formulations (1)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Applications

Clonality

Clone number

Isotype

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

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