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Suitable for WB, ELISA. Cited in 2 publications.

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Images

Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (AB99696), expandable thumbnail
  • Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (AB99696), expandable thumbnail

Publications

Key facts

Host species

Mouse

Target species

Rabbit

Target isotype

IgG

Target specificity

Light chain

Conjugation

Alkaline Phosphatase

Applications

WB, ELISA

Clonality

Monoclonal

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/2000.00000 - 1/4000.00000

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Recommended products

Suitable for WB, ELISA. Cited in 2 publications.

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Key facts

Description

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)

Host species

Mouse

Target species

Rabbit

Target isotype

IgG

Target specificity

Light chain

Conjugation

Alkaline Phosphatase

Applications

WB, ELISA

Clonality

Monoclonal

Clone number

SB62a

Pre-adsorbed

No

Specificity

ab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD).

Isotype

IgG2b

Light chain type

lambda

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

pH: 8
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.605% Tris, 0.0095% Magnesium chloride

Purification technique

Affinity purification Protein A

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696), expandable thumbnail

    Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

    Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains

    Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)

    Lane 1: 10 μg rabbit monoclonal was loaded
    Lane 2: 5 μg rabbit monoclonal was loaded
    Lane 3: 2 μg rabbit monoclonal was loaded

    Right blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000

    Lane 1: 1 μg rabbit monoclonal was loaded
    Lane 2: 0.5 μg rabbit monoclonal was loaded
    Lane 3: 0.25 μg rabbit monoclonal was loaded

    Secondary

    All lanes: Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

  • Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696), expandable thumbnail

    Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

    All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1 μg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 μg per lane.

    Secondary
    Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
    Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
    Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Secondary

    All lanes: Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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