Rabbit Anti-Armenian hamster IgG H&L (HRP)

• WB

Supplier Data

Western blot - Rabbit Anti-Armenian hamster IgG H&L (HRP) (AB5745)

Blocking/diluting buffer and concentration : 5% NFDM/TBST. Upregulating the expression of TPN protein by IFN gamma treatment (PMID : 11169257). Exposure time : 3.25 seconds.

All lanes:

Western blot - Anti-TPN antibody [5D3] (<a href='/en-us/products/primary-antibodies/tpn-antibody-5d3-ab307298'>ab307298</a>) at 1/1000 dilution

Lane 1:

Untreated B16-F10 (Mouse skin melanoma), whole cell lysate 20 μg

Lane 2:

B16-F10 treated with /ml IFN gamma for 16 hours, whole cell lysate 20 μg

Observed band size: 48 kDa

false

Exposure time: 3.25s

• ELISA

Supplier Data

ELISA - Rabbit Anti-Armenian hamster IgG H&L (HRP) (AB5745)

ab5745 tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 μg of Armenian Hamster IgG. The starting dilution of antibody was 5μg/ml and the X-axis represents the log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate

• WB

Lab

Western blot - Rabbit Anti-Armenian hamster IgG H&L (HRP) (AB5745)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11384980).

Lysates should be made freshly and used in WB immediately.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1/2：3 seconds; Lane 3：15 seconds; Lane 4：37 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243248).

All lanes:

Western blot - Anti-p115-RhoGEF antibody [JH-1] (<a href='/en-us/products/primary-antibodies/p115-rhogef-antibody-jh-1-ab243248'>ab243248</a>) at 1/1000 dilution

Lane 1:

A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 10 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 4:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Armenian hamster IgG H&L (HRP) (ab5745) at 1/5000 dilution

Predicted band size: 102 kDa

Observed band size: 115 kDa

false

• Dot

Supplier Data

Dot Blot - Rabbit Anti-Armenian hamster IgG H&L (HRP) (AB5745)

Dot blot analysis of CD30 using ab324907 at 1 : 1000 (0.998 ug/ml) followed by a Rabbit Anti-Armenian hamster IgG H&L (HRP) (ab5745) at 1 : 2000 dilution.

Lane1 : Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein

Exposure time : 10 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-CD30 antibody [mCD30.1] (<a href='/en-us/products/primary-antibodies/cd30-antibody-mcd301-ab324907'>ab324907</a>) at 1/1000 dilution

All lanes:

Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein

Secondary

All lanes:

Dot Blot - Rabbit Anti-Armenian hamster IgG H&L (HRP) (ab5745) at 1/2000 dilution

false

Exposure time: 10s