Suitable for ICC, IHC-P, ELISA, WB. Cited in 2 publications.
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Publications
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- Archives of oral biology 110:1046212019Effects of spaceflight on the mouse submandibular gland.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArthur R Hand,Didem Dagdeviren,Natasha A Larson,Christopher Haxhi,Maija I MednieksPubMed 31805482
- Molecular and cellular endocrinology 441:116-1232016Genes and proteins of the alternative steroid backdoor pathway for dihydrotestosterone synthesis are expressed in the human ovary and seem enhanced in the polycystic ovary syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNesa Marti,José A Galván,Amit V Pandey,Mafalda Trippel,Coya Tapia,Michel Müller,Aurel Perren,Christa E FlückPubMed 27471004
Key facts
- Host species
- Rabbit
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/100.00000 - 1/30000.00000 | Notes Coating: Use 1/100 - 1/500, Primary: Use 1/1000 - 1/30000 |
Application WB | Reactivity Reacts | Dilution info 1/1000.00000 - 1/30000.00000 | Notes Colorimetric: Use 1/1000 - 10000, Chemiluminescent: Use 1/1000 - 1/30000 |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ICC, IHC-P, ELISA, WB. Cited in 2 publications.
Key facts
- Description
- Rabbit Anti-Chicken IgY H&L
- Host species
- Rabbit
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (ab97136)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum.
The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (ab97136)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum.
The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (ab97136)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum.
The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (ab97136)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse skeletal muscle
The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (ab97136)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse kidney.
The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
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