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AB97136

Rabbit Anti-Chicken IgY H&L

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(2 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Cited in 2 publications.
5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MAP2 with ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebrum.
The section was incubated with ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MAP2 with ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse kidney.
The section was incubated with ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MAP2 with ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse skeletal muscle
The section was incubated with ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling MAP2 with ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebrum.
The section was incubated with ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Chicken IgY H&L (AB97136)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling MAP2 with ab318993 at 1/50000 (0.019 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum.
The section was incubated with ab318993 for 10 mins at room temperature and followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins

Key facts

Host species

Rabbit

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Applications

WB, ICC, ELISA, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/30000", "notes":"<p>Coating: Use 1/100 - 1/500, Primary: Use 1/1000 - 1/30000</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/30000", "notes":"<p>Colorimetric: Use 1/1000 - 10000, Chemiluminescent: Use 1/1000 - 1/30000</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Archives of oral biology 110:104621 PubMed31805482

2019

Effects of spaceflight on the mouse submandibular gland.

Applications

Unspecified application

Species

Unspecified reactive species

Arthur R Hand,Didem Dagdeviren,Natasha A Larson,Christopher Haxhi,Maija I Mednieks

Molecular and cellular endocrinology 441:116-123 PubMed27471004

2016

Genes and proteins of the alternative steroid backdoor pathway for dihydrotestosterone synthesis are expressed in the human ovary and seem enhanced in the polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Nesa Marti,José A Galván,Amit V Pandey,Mafalda Trippel,Coya Tapia,Michel Müller,Aurel Perren,Christa E Flück
View all publications

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