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AB6752

Rabbit Anti-Chicken IgY H&L (Biotin)

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(10 Publications)

Rabbit Anti-Chicken IgY H&L (Biotin)(ab6752) is a biotin conjugated secondary antibody that is suitable for Western blot, IHC, ELISA and more.

- Suitable for avidin-biotin complex (ABC) method or labeled streptavidin-biotin (LSAB) method
2 Images
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (AB6752)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (AB6752)

ab6752 was used with the primary antibody ab13989 in ICC/IF. See Abreview on ab13989.

Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (AB6752)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (AB6752)

ab6752 was used at dilution 1/1000 with the primary antibody ab13970 in IHC-Fr. See the review on ab13970.

Key facts

Host species

Rabbit

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

WB, ICC/IF, ELISA, IHC-P, Dot, IM, IHC-Fr

applications

Clonality

Polyclonal

Specificity

Some light chain cross reactivity may be observed against chicken immunoglobulins.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500000", "notes":"<p></p>" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/20000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Biotin Conjugated Anti-Chicken IgG / IgY secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG / IgY coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Chicken IgY
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Tissue engineering. Part A 26:993-1004 PubMed32122263

2020

Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model.

Applications

Unspecified application

Species

Unspecified reactive species

Alba López-Fernández,Víctor Barro,Mònica Ortiz-Hernández,Maria Cristina Manzanares,Daniel Vivas,Joaquim Vives,Roberto Vélez,Maria Pau Ginebra,Màrius Aguirre

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 31:882-892 PubMed27864378

2016

Loss of scleraxis in mice leads to geometric and structural changes in cortical bone, as well as asymmetry in fracture healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A McKenzie,Evan Buettmann,Adam C Abraham,Michael J Gardner,Matthew J Silva,Megan L Killian

Journal of tissue engineering and regenerative medicine 11:3408-3416 PubMed27860364

2016

A reproducible method for the isolation and expansion of ovine mesenchymal stromal cells from bone marrow for use in regenerative medicine preclinical studies.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Caminal,Roberto Vélez,Rosa Maria Rabanal,Daniel Vivas,Laura Batlle-Morera,Màrius Aguirre,Jordi Barquinero,Joan García,Joaquim Vives

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 30:612-23 PubMed26443823

2015

Imaging rhodopsin degeneration in vivo in a new model of ocular ischemia in living mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqian Ren,Yinching I Chen,Ashley M Mackey,Philip K Liu

Oncogene 34:1822-30 PubMed24837369

2014

Inhibition of intestinal tumor formation by deletion of the DNA methyltransferase 3a.

Applications

Unspecified application

Species

Unspecified reactive species

B Weis,J Schmidt,H Maamar,A Raj,H Lin,C Tóth,K Riedmann,G Raddatz,H-K Seitz,A D Ho,F Lyko,H G Linhart

International journal of clinical and experimental 6:3036-41 PubMed24294399

2013

Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer.

Applications

IHC-P

Species

Unspecified reactive species

Ting-Ting Jiao,Ye-Min Zhang,Lin Yao,Yuan Gao,Jian Sun,Dong-Fang Zou,Guo-Ping Wu,Dan Wang,Jun Ou,Ning Hui

World journal of urology 29:801-6 PubMed21113600

2010

XPA-210: a new proliferation marker to characterize tumor biology and progression of renal cell carcinoma.

Applications

IHC-P

Species

Unspecified reactive species

Georgios Gakis,Joerg Hennenlotter,Marcus Scharpf,Joachim Hevler,David Schilling,Ursula Kuehs,Arnulf Stenzl,Christian Schwentner

Proceedings of the National Academy of Sciences of 107:11399-404 PubMed20534521

2010

Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event.

Applications

IHC-Fr

Species

Unspecified reactive species

Elissa W P Wong,Dolores D Mruk,Will M Lee,C Yan Cheng

Respiratory research 9:32 PubMed18423037

2008

Characterization of a panel of six beta2-adrenergic receptor antibodies by indirect immunofluorescence microscopy.

Applications

ICC/IF

Species

Unspecified reactive species

Yulia A Koryakina,Tristan W Fowler,Stacie M Jones,Bradley J Schnackenberg,Lawrence E Cornett,Richard C Kurten

The Journal of biological chemistry 279:27753-63 PubMed15056671

2004

Cell type-specific expression of LINE-1 open reading frames 1 and 2 in fetal and adult human tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Süleyman Ergün,Christian Buschmann,Jochen Heukeshoven,Kristin Dammann,Frank Schnieders,Heidrun Lauke,Fariba Chalajour,Nerbil Kilic,Wolf H Strätling,Gerald G Schumann
View all publications
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Product promise

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