Suitable for Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF. Ideal for western blot. Cited in 28 publications.
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Publications
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- The Journal of biological chemistry 300:1072632024JNK activity modulates postsynaptic scaffold protein SAP102 and kainate receptor dynamics in dendritic spines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStella-Amrei Kunde,Bettina Schmerl,Judith von Sivers,Elham Ahmadyar,Taanisha Gupta,Nils Rademacher,Hanna L Zieger,Sarah A ShoichetPubMed 38582451
- Journal of the Endocrine Society 6:bvac1412022Mice With RIP-Cre-mediated Deletion of the Long Noncoding RNA Show Normal Pancreatic Islets and Enlarged Pituitary.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVaishali I Parekh,Hui Sun,Min Chen,Lee S Weinstein,Sunita K AgarwalPubMed 37283960
- PLoS biology 20:e30015032022The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBettina Schmerl,Niclas Gimber,Benno Kuropka,Alexander Stumpf,Jakob Rentsch,Stella-Amrei Kunde,Judith von Sivers,Helge Ewers,Dietmar Schmitz,Christian Freund,Jan Schmoranzer,Nils Rademacher,Sarah A ShoichetPubMed 35312684
- Microorganisms 10:2022Transformation of by for the Expression of the Hemagglutinin of Avian Influenza Virus H5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInkar Castellanos-Huerta,Gabriela Gómez-Verduzco,Guillermo Tellez-Isaias,Guadalupe Ayora-Talavera,Bernardo Bañuelos-Hernández,Víctor Manuel Petrone-García,Gilberto Velázquez-Juárez,Isidro Fernández-SiurobPubMed 35208815
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:20049292021Delivering Antisense Oligonucleotides across the Blood-Brain Barrier by Tumor Cell-Derived Small Apoptotic Bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulian Wang,Jiayun Pang,Qingyun Wang,Luocheng Yan,Lintao Wang,Zhen Xing,Chunming Wang,Junfeng Zhang,Lei DongPubMed 34258157
- Vaccines 8:2020Anti-S1 MERS-COV IgY Specific Antibodies Decreases Lung Inflammation and Viral Antigen Positive Cells in the Human Transgenic Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAymn T Abbas et. al.PubMed 33139631
- Nature communications 11:31402020MeCP2 regulates gene expression through recognition of H3K27me3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWooje Lee et. al.PubMed 32561780
- Clinical epigenetics 11:1022019Apabetalone (RVX-208) reduces vascular inflammation in vitro and in CVD patients by a BET-dependent epigenetic mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura M Tsujikawa et. al.PubMed 31300040
- Endocrine-related cancer 25:L31-L352018Frequency and consequence of the recurrent p.T372R mutation in sporadic insulinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVaishali I Parekh,Sita D Modali,James Welch,William F Simonds,Lee S Weinstein,Electron Kebebew,Sunita K AgarwalPubMed 29467239
- Endocrinology 159:1199-12122018Functional Defects From Endocrine Disease-Associated Mutations in HLXB9 and Its Interacting Partner, NONO.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSampada S Kharade,Vaishali I Parekh,Sunita K AgarwalPubMed 29309627
Key facts
- Host species
- Rabbit
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info 1/2500 - 1/10000 | Notes - |
Application ELISA | Reactivity Expected | Dilution info 1/50000 - 1/100000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF. Ideal for western blot. Cited in 28 publications.
Key facts
- Description
- Rabbit Anti-Chicken IgY H&L (HRP)
- Host species
- Rabbit
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
Some light chain cross reactivity may be observed against chicken immunoglobulins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
Anti-Chicken IgG/IgY Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
May cross-react with chicken IgM
Product promise
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3 product images
This image is courtesy of an anonymous customer reviewWestern blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
All lanes: Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody ab1170) at 1/5000 dilution
All lanes: Escherichia coli Recombinant protein (BL21)
SecondaryAll lanes: Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 kDa
Exposure time: 10s
ELISA - Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
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