AB6753

Rabbit Anti-Chicken IgY H&L (HRP)

Name: Rabbit Anti Chicken (IgY) secondary antibody HRP (ab6753) | Abcam
Brand: Abcam Limited
SKU: AB6753

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(32 Publications)

Suitable for Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC. Ideal for western blot. Cited in 32 publications.

4 Images

AbReview15737****

Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

All lanes:

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (<a href='/en-us/products/primary-antibodies/ddddk-tag-binds-to-flag-tag-sequence-antibody-ab1170'>ab1170</a>) at 1/5000 dilution

All lanes:

Escherichia coli Recombinant protein (BL21)

Secondary

All lanes:

Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution

Observed band size: 66 kDa

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Exposure time: 10s

This image is courtesy of an anonymous Abreview

Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

ab6753 was used at dilution 1/2000 with the primary antibody ab16293 in IHC-Fr. See the review on ab16293.

ELISA - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

ELISA

Unknown

ELISA - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

ab6753 was used at dilution 1/10000 with the primary antibody ab15879 in ELISA. See the review on ab15879.

Unknown

Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

ab6753 was used at dilution 1/5000 with the primary antibody ab19130 in WB. See the review on ab19130.

Secondary

All lanes:

Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)

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Key facts

Host species

Rabbit

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IHC-Fr, IM, ELISA, WB, ICC, Dot, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

Some light chain cross reactivity may be observed against chicken immunoglobulins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2500 - 1/10000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50000 - 1/100000", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Product details

May cross-react with chicken IgM

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-Chicken IgG/IgY Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Chicken IgY

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 19:1656777 PubMed40969613

2025

Early olfactory dysfunction in experimental autoimmune encephalomyelitis reflects transient brain barrier breach and initiation of neuroinflammation in the olfactory bulb.

Applications

Unspecified application

Species

Unspecified reactive species

Andjela Stekic,Milorad Dragic,Ivana Stevanovic,Marina Zaric Kontic,Marija Adzic Bukvic,Sanja Dacic,Milica Ninkovic,Nadezda Nedeljkovic

Environment international 190:108838 PubMed38963985

2024

The pollutant perfluorohexane sulfonate (PFHxS) reduces serum thyroxine but does not alter thyroid action in the postnatal rat brain.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine L O'Shaughnessy,Kiersten S Bell,Aubrey L Sasser,Mary E Gilbert,Cal Riutta,Jermaine L Ford,James McCord,Carmen R Wood

Vaccines 12: PubMed38675777

2024

Evaluation of a FlpA Glycoconjugate Vaccine with Ten -Heptasaccharide Glycan Moieties to reduce Colonisation in Chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Corona-Torres,Prerna Vohra,Cosmin Chintoan-Uta,Abi Bremner,Vanessa S Terra,Marta Mauri,Jon Cuccui,Lonneke Vervelde,Brendan W Wren,Mark P Stevens

The Journal of biological chemistry 300:107263 PubMed38582451

2024

JNK activity modulates postsynaptic scaffold protein SAP102 and kainate receptor dynamics in dendritic spines.

Applications

Unspecified application

Species

Unspecified reactive species

Stella-Amrei Kunde,Bettina Schmerl,Judith von Sivers,Elham Ahmadyar,Taanisha Gupta,Nils Rademacher,Hanna L Zieger,Sarah A Shoichet

Journal of the Endocrine Society 6:bvac141 PubMed37283960

2022

Mice With RIP-Cre-mediated Deletion of the Long Noncoding RNA Show Normal Pancreatic Islets and Enlarged Pituitary.

Applications

Unspecified application

Species

Unspecified reactive species

Vaishali I Parekh,Hui Sun,Min Chen,Lee S Weinstein,Sunita K Agarwal

PLoS biology 20:e3001503 PubMed35312684

2022

The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Bettina Schmerl,Niclas Gimber,Benno Kuropka,Alexander Stumpf,Jakob Rentsch,Stella-Amrei Kunde,Judith von Sivers,Helge Ewers,Dietmar Schmitz,Christian Freund,Jan Schmoranzer,Nils Rademacher,Sarah A Shoichet

Microorganisms 10: PubMed35208815

2022

Transformation of by for the Expression of the Hemagglutinin of Avian Influenza Virus H5.

Applications

Unspecified application

Species

Unspecified reactive species

Inkar Castellanos-Huerta,Gabriela Gómez-Verduzco,Guillermo Tellez-Isaias,Guadalupe Ayora-Talavera,Bernardo Bañuelos-Hernández,Víctor Manuel Petrone-García,Gilberto Velázquez-Juárez,Isidro Fernández-Siurob

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:2004929 PubMed34258157

2021

Delivering Antisense Oligonucleotides across the Blood-Brain Barrier by Tumor Cell-Derived Small Apoptotic Bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Yulian Wang,Jiayun Pang,Qingyun Wang,Luocheng Yan,Lintao Wang,Zhen Xing,Chunming Wang,Junfeng Zhang,Lei Dong

Vaccines 8: PubMed33139631

2020

Anti-S1 MERS-COV IgY Specific Antibodies Decreases Lung Inflammation and Viral Antigen Positive Cells in the Human Transgenic Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Aymn T Abbas,Sherif A El-Kafrawy,Sayed Sartaj Sohrab,Ashraf A Tabll,Ahmed M Hassan,Naoko Iwata-Yoshikawa,Noriyo Nagata,Esam I Azhar

Nature communications 11:3140 PubMed32561780

2020

MeCP2 regulates gene expression through recognition of H3K27me3.

Applications

Unspecified application

Species

Unspecified reactive species

Wooje Lee,Jeeho Kim,Jung-Mi Yun,Takbum Ohn,Qizhi Gong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rabbit Anti-Chicken IgY H&L (HRP)

Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

ELISA - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (AB6753)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (32)

Product promise