AB97140

Rabbit Anti-Chicken IgY H&L (HRP)

Name: Rabbit Anti Chicken (IgY) secondary antibody HRP (ab97140) | Abcam
Brand: Abcam Limited
SKU: AB97140
Price: 265 USD
Availability: InStock

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(8 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 8 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images

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Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (AB97140)

All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 μg/ml

Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 μg per lane.

Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/2000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/20000 dilution

Developed using the ECL technique

Performed under reducing conditions.

Exposure time : 1 minute

Secondary

All lanes:

Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab97140)

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Key facts

Host species

Rabbit

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IHC-P, WB, ELISA, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"(Primary)." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/20000", "notes":"Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000" } } }

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Product details

Part of the AbExcel range.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Chicken IgY

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 53:98-105 PubMed40342119

2025

Knockdown of TRIM22 regulates the expression of NF-κB/NLRP3 and alleviates inflammation and renal injury in mice with lupus nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Lu Liu,Min Liu,Mingming Qin,Suyan Yan,Chunfeng Wu,Wenjie Yu

Vaccines 10: PubMed35214684

2022

Efficacy of Multivalent, Cochleate-Based Vaccine against Infantis, Enteritidis and Typhimurium in Laying Hens.

Applications

Unspecified application

Species

Unspecified reactive species

Leonardo Sáenz,Miguel Guzmán,Sonia Vidal,Mario Caruffo,Daniela Siel,Caridad Zayas,Rodolfo Paredes,Carolina Valenzuela,Héctor Hidalgo,Oliver Pérez,Lisette Lapierre

Nanomaterials (Basel, Switzerland) 10: PubMed33233490

2020

An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic via Both Humoral and Predominantly Th1 Immune Responses in Poultry.

Applications

Unspecified application

Species

Unspecified reactive species

Rujiu Hu,Haojing Liu,Mimi Wang,Jing Li,Hua Lin,Mingyue Liang,Yupeng Gao,Mingming Yang

Molecules (Basel, Switzerland) 24: PubMed31387223

2019

Dietary Curcumin Prevented Astrocytosis, Microgliosis, and Apoptosis Caused by Acute and Chronic Exposure to Ozone.

Applications

Unspecified application

Species

Unspecified reactive species

Sendar Daniel Nery-Flores,Mario Alberto Ramírez-Herrera,María Luisa Mendoza-Magaña,Marina María de Jesús Romero-Prado,José de Jesús Ramírez-Vázquez,Jacinto Bañuelos-Pineda,Hugo Alejandro Espinoza-Gutiérrez,Abraham Alberto Ramírez-Mendoza,Mariana Chávez Tostado

The Journal of general physiology 149:149-170 PubMed27908976

2016

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.

Applications

Unspecified application

Species

Unspecified reactive species

Anne C Wolfes,Saheeb Ahmed,Ankit Awasthi,Markus A Stahlberg,Ashish Rajput,Daniel S Magruder,Stefan Bonn,Camin Dean

Journal of cell science 128:3583-96 PubMed26290381

2015

Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex.

Applications

Unspecified application

Species

Unspecified reactive species

Nina K Thiede-Stan,Björn Tews,David Albrecht,Zorica Ristic,Helge Ewers,Martin E Schwab

The Journal of biological chemistry 290:5685-95 PubMed25561728

2015

Mechanism-based proteomic screening identifies targets of thioredoxin-like proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Lia S Nakao,Robert A Everley,Stefano M Marino,Sze M Lo,Luiz E de Souza,Steven P Gygi,Vadim N Gladyshev

International journal of cancer 134:530-41 PubMed23873303

2013

Evidence for the antagonistic form of CXC-motif chemokine CXCL10 in serous epithelial ovarian tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Rainczuk,Jyothsna R Rao,Jessica L Gathercole,Nicole J Fairweather,Simon Chu,Rina Masadah,Thomas W Jobling,Santanu Deb-Choudhury,Jolon Dyer,Andrew N Stephens

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rabbit Anti-Chicken IgY H&L (HRP)

Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (AB97140)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (8)

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise