Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 7 publications.
Images
Publications
Filter by
- Vaccines 10:2022Efficacy of Multivalent, Cochleate-Based Vaccine against Infantis, Enteritidis and Typhimurium in Laying Hens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonardo Sáenz,Miguel Guzmán,Sonia Vidal,Mario Caruffo,Daniela Siel,Caridad Zayas,Rodolfo Paredes,Carolina Valenzuela,Héctor Hidalgo,Oliver Pérez,Lisette LapierrePubMed 35214684
- Nanomaterials (Basel, Switzerland) 10:2020An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic via Both Humoral and Predominantly Th1 Immune Responses in Poultry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRujiu Hu et. al.PubMed 33233490
- Molecules (Basel, Switzerland) 24:2019Dietary Curcumin Prevented Astrocytosis, Microgliosis, and Apoptosis Caused by Acute and Chronic Exposure to Ozone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSendar Daniel Nery-Flores et. al.PubMed 31387223
- The Journal of general physiology 149:149-1702016A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne C Wolfes,Saheeb Ahmed,Ankit Awasthi,Markus A Stahlberg,Ashish Rajput,Daniel S Magruder,Stefan Bonn,Camin DeanPubMed 27908976
- Journal of cell science 128:3583-962015Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNina K Thiede-Stan et. al.PubMed 26290381
- The Journal of biological chemistry 290:5685-952015Mechanism-based proteomic screening identifies targets of thioredoxin-like proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLia S Nakao et. al.PubMed 25561728
- International journal of cancer 134:530-412013Evidence for the antagonistic form of CXC-motif chemokine CXCL10 in serous epithelial ovarian tumours.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Rainczuk,Jyothsna R Rao,Jessica L Gathercole,Nicole J Fairweather,Simon Chu,Rina Masadah,Thomas W Jobling,Santanu Deb-Choudhury,Jolon Dyer,Andrew N StephensPubMed 23873303
Key facts
- Host species
- Rabbit
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200 - 1/5000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000 - 1/100000 | Notes (Primary). |
Application WB | Reactivity Reacts | Dilution info 1/2000 - 1/20000 | Notes Colorimetric: Use 1/5,000-1/30,000, Chemiluminescent: Use 1/10,000-1/50,000 |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 7 publications.
Key facts
- Description
- Rabbit Anti-Chicken IgY H&L (HRP)
- Host species
- Rabbit
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ICC, IHC-P, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Part of the AbExcel range.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab97140)
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 μg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 μg per lane.
Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/2000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97140) at 1/20000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minuteSecondaryAll lanes: Western blot - Rabbit Anti-Chicken IgY H&L (HRP) (ab97140)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com