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Suitable for FuncS, ELISA, IHC-P, WB, IHC-Fr, ICC.

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Key facts

Host species
Rabbit
Target species
Dog
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
Alkaline Phosphatase
Applications
FuncS, ELISA, IHC-P, WB, IHC-Fr, ICC
Clonality
Polyclonal
Format
F(ab')2

Reactivity data

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/500 - 1/5000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/25 - 1/100
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/100 - 1/1000
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
1/25 - 1/100
Notes

-

Application
ICC
Reactivity
Reacts
Dilution info
1/25 - 1/100
Notes

-

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Recommended products

Suitable for FuncS, ELISA, IHC-P, WB, IHC-Fr, ICC.

Key facts

Description
Rabbit F(ab')2 Anti-Dog IgG H&L (Alkaline Phosphatase)
Host species
Rabbit
Target species
Dog
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
Alkaline Phosphatase
Applications
FuncS, ELISA, IHC-P, WB, IHC-Fr, ICC
Clonality
Polyclonal
Format
F(ab')2
Pre-adsorbed
No
Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48.9% Tris buffered saline, 1% BSA

Purification technique
Affinity purification Immunogen
Purification notes

ab136764 is purified by affinity chromatography and is thus particularly specific.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Preparation of Substrate Solutions:


ELISA : 1M diethanolamin, 0.5mM MgCl2, 15mM pNPP, pH 9.8. Incubate for 15 minutes at 37°C. Stop the reaction with V/5 K2HP04 2M. Read at 405 nm.


Immunoblot : 34 µl of BCIP (5bromo-4chloroindolylphosphate) at 50mg/ml in DMF + 44 µl NBT (NitroBlue Tetrazolium) at 75 mg/ml in DMF 70% + 10ml substrate buffer (0.1M TRIS/HCI, 0.5M NaCl, 0.05M MgCl2, pH 9.5).

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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