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AB136759

Rabbit F(ab')2 Anti-Dog IgG H&L (HRP)

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(1 Publication)

Suitable for Flow Cyt, ELISA, IHC-P, WB, IHC-Fr, ICC. Ideal for western blot. Cited in 1 publication.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Rabbit

Target species

Dog

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

HRP

Excitation/Emission
Applications

IHC-Fr, ICC, ELISA, Flow Cyt, IHC-P, WB

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p></p>" } } }
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Product details

Preparation of Substrate Solutions:

- ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm
- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab136759 is purified by affinity chromatography and is thus particularly specific.
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 50% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information Dog IgG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Infection and immunity 86: PubMed29229735

2018

Oral Application of Recombinant Bacillus subtilis Spores to Dogs Results in a Humoral Response against Specific Echinococcus granulosus Paramyosin and Tropomyosin Antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Cédric M Vogt,Maria Teresa Armúa-Fernández,Kurt Tobler,Monika Hilbe,Claudio Aguilar,Mathias Ackermann,Peter Deplazes,Catherine Eichwald
View all publications
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