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Suitable for Flow Cyt, ELISA, IHC-P, WB, IHC-Fr, ICC. Ideal for western blot. Cited in 1 publication.

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Key facts

Host species
Rabbit
Target species
Dog
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
Flow Cyt, ELISA, IHC-P, WB, IHC-Fr, ICC
Clonality
Polyclonal
Format
F(ab')2

Reactivity data

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/500 - 1/5000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/20 - 1/100
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/100 - 1/1000
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
1/20 - 1/100
Notes

-

Application
ICC
Reactivity
Reacts
Dilution info
1/20 - 1/100
Notes

-

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Recommended products

Suitable for Flow Cyt, ELISA, IHC-P, WB, IHC-Fr, ICC. Ideal for western blot. Cited in 1 publication.

Key facts

Description
Rabbit F(ab')2 Anti-Dog IgG H&L (HRP)
Host species
Rabbit
Target species
Dog
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
Flow Cyt, ELISA, IHC-P, WB, IHC-Fr, ICC
Clonality
Polyclonal
Format
F(ab')2
Pre-adsorbed
No
Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine), 50% Tris buffered saline

Purification technique
Affinity purification Immunogen
Purification notes

ab136759 is purified by affinity chromatography and is thus particularly specific.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Preparation of Substrate Solutions:



- ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm


- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.


- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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