AB5755

Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed

Name: Rabbit F(ab')2 Anti Goat (IgG) secondary antibody preadsorbed HRP (ab5755)
Brand: Abcam Limited
SKU: AB5755

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(3 Publications)

Suitable for Dot, EM, IHC, ICC, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

IHC, ICC, ELISA, Dot, WB, EM

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 21:289 PubMed33717232

2021

Poricoic acid A suppresses TGF-β1-induced renal fibrosis and proliferation via the PDGF-C, Smad3 and MAPK pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Li,Yao Ming,Hu Jia,Gang Wang

Scientific reports 7:10180 PubMed28860510

2017

A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

David Díaz-Jiménez,Lucía Núñez,Marjorie De la Fuente,Karen Dubois-Camacho,Hugo Sepúlveda,Martín Montecino,Alejandro Torres-Riquelme,Paulina García-González,Jonás Chnaiderman,Anna Vossenkamper,Thomas T MacDonald,Daniela Simian,María-Julieta González,John A Cidlowski,Rodrigo Quera,Marcela A Hermoso

PloS one 10:e0125384 PubMed26047103

2015

Forward Programming of Cardiac Stem Cells by Homogeneous Transduction with MYOCD plus TBX5.

Applications

Species

Unspecified reactive species

Elisa Belian,Michela Noseda,Marta S Abreu Paiva,Thomas Leja,Robert Sampson,Michael D Schneider

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit F(ab')2 Anti-Goat IgG - H&L (HRP), pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (3)

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise