Suitable for ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt, Dot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
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Publications
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- American journal of translational research 13:2111-21262021NEAT1 promotes the repair of abdominal aortic aneurysms of endothelial progenitor cells via regulating miR-204-5p/Ang-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Shi,Chongwen Shen,Ge Liu,Shaofeng Yang,Fenglin Ye,Jinjin Meng,Youmin PanPubMed 34017378
- Molecular therapy oncolytics 20:187-1982021IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinlu Tian,Hongxue Meng,Xiao Dong,Xinlei Li,Zilin Shi,Hulun Li,Lie Zhang,Yue Yang,Ruijie Liu,Chunying Pei,Bo Li,Hongwei Xu,Rui LiPubMed 33665357
- Reproductive biomedicine online 37:184-2002018Distinct changes in the proteome profile of endometrial tissues in polycystic ovary syndrome compared with healthy fertile women.Applications:IHC-P, WBReactive species:Unspecified reactive speciesFatemehsadat Amjadi et. al.PubMed 29729850
- Molecular medicine reports 17:7902-79102018Differentiation of genetically modified canine bone mesenchymal stem cells labeled with superparamagnetic iron oxide into neural‑like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXing-Long Liu et. al.PubMed 29620288
- Oncology reports 39:687-6942017Isoliquiritigenin inhibits the proliferation and induces the differentiation of human glioma stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuliang Lin,Hongjun Sun,Ying Dang,Zhiyun LiPubMed 29251326
Key facts
- Host species
- Rabbit
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Human
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt, Dot
- Clonality
- Polyclonal
- Format
- F(ab')2
Immunogen
- Database link P01868
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Expected | Dilution info 1/100 - 1/250 | Notes - |
Application IHC-P | Reactivity Expected | Dilution info 1/50 - 1/200 | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info 1/50 - 1/200 | Notes - |
Application IM | Reactivity Expected | Dilution info 1/50 - 1/200 | Notes - |
Application Flow Cyt | Reactivity Expected | Dilution info 1/100 - 1/250 | Notes Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate. |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt, Dot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
Key facts
- Description
- Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed
- Host species
- Rabbit
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Human
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt, Dot
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Immunogen
- Database link P01868
- Specificity
No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Dot Blot - Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)
Dot blot analysis of ab7000. Imaged with BioRad ChemiDoc, Rhodamine filter.
All lanes: Dot Blot - Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)
Lane 1: Mouse IgG at 100 ng
Lane 2: Mouse IgG at 33.3 ng
Lane 3: Mouse IgG at 11.1 ng
Lane 4: Mouse IgG at 3.7 ng
Lane 5: Mouse IgG at 1.23 ng
SecondaryAll lanes: Rabbit F(ab')2 Anti-Mouse IgG Antibody RPE at 1 µg/mL
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