AB7000

Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed

Name: Rabbit F(ab')2 Anti Mouse (IgG) secondary antibody preadsorbed PE (ab7000)
Brand: Abcam Limited
SKU: AB7000
Price: 535 USD
Availability: InStock

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(5 Publications)

Suitable for ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt, Dot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

Supplier Data

Dot Blot - Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (AB7000)

Dot blot analysis of ab7000. Imaged with BioRad ChemiDoc, Rhodamine filter.

All lanes:

Dot Blot - Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (ab7000)

Lane 1:

Mouse IgG at 100 ng

Lane 2:

Mouse IgG at 33.3 ng

Lane 3:

Mouse IgG at 11.1 ng

Lane 4:

Mouse IgG at 3.7 ng

Lane 5:

Mouse IgG at 1.23 ng

Secondary

All lanes:

Rabbit F(ab')2 Anti-Mouse IgG Antibody RPE at 1 µg/mL

false

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Human

Pre-adsorbed

Yes

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

IHC-P, IHC-Fr, Dot, IM, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Specificity

No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/250", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"" }, "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/250", "notes":"Use 1 x 106 cells in flow cytometry is approximately 1.0 mg of antibody conjugate." }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Product details

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 13:2111-2126 PubMed34017378

2021

NEAT1 promotes the repair of abdominal aortic aneurysms of endothelial progenitor cells via regulating miR-204-5p/Ang-1.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Shi,Chongwen Shen,Ge Liu,Shaofeng Yang,Fenglin Ye,Jinjin Meng,Youmin Pan

Molecular therapy oncolytics 20:187-198 PubMed33665357

2021

IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application.

Applications

Unspecified application

Species

Unspecified reactive species

Linlu Tian,Hongxue Meng,Xiao Dong,Xinlei Li,Zilin Shi,Hulun Li,Lie Zhang,Yue Yang,Ruijie Liu,Chunying Pei,Bo Li,Hongwei Xu,Rui Li

Reproductive biomedicine online 37:184-200 PubMed29729850

2018

Distinct changes in the proteome profile of endometrial tissues in polycystic ovary syndrome compared with healthy fertile women.

Applications

IHC-P, WB

Species

Unspecified reactive species, Unspecified reactive species

Fatemehsadat Amjadi,Mehdi Mehdizadeh,Mahnaz Ashrafi,Davood Nasrabadi,Sara Taleahmad,Mehdi Mirzaei,Vivek Gupta,Ghasem Hosseini Salekdeh,Reza Aflatoonian

Molecular medicine reports 17:7902-7910 PubMed29620288

2018

Differentiation of genetically modified canine bone mesenchymal stem cells labeled with superparamagnetic iron oxide into neural‑like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Long Liu,Qing-Quan Zu,Bin Wang,Shan-Shan Lu,Xiao-Quan Xu,Sheng Liu,Hai-Bin Shi

Oncology reports 39:687-694 PubMed29251326

2017

Isoliquiritigenin inhibits the proliferation and induces the differentiation of human glioma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuliang Lin,Hongjun Sun,Ying Dang,Zhiyun Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed

Dot Blot - Rabbit F(ab')2 Anti-Mouse IgG - H&L (PE), pre-adsorbed (AB7000)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (5)

Product promise