AB136761
Rabbit F(ab')2 Anti-Pig IgG H&L (HRP)
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Suitable for Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA. Ideal for western blot.
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Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20 - 1/100", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"<p></p>" } } }
Product details
Preparation of Substrate Solutions:
- ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm
- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.
- ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm
- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.
Properties and storage information
Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab136761 is purified by affinity chromatography and is thus particularly specific.
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 50% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Target data
Product promise
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