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Suitable for Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA. Ideal for western blot.

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Key facts

Host species

Rabbit

Target species

Pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA

Clonality

Polyclonal

Format

F(ab')2

Reactivity data

Application

Flow Cyt

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ICC

Reactivity

Reacts

Dilution info

1/20 - 1/100

Notes

-

Application

IHC-Fr

Reactivity

Reacts

Dilution info

1/20 - 1/100

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

1/100 - 1/1000

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

1/20 - 1/100

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/500 - 1/5000

Notes

-

Alternative names

Recommended products

Suitable for Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA. Ideal for western blot.

Key facts

Description

Rabbit F(ab')2 Anti-Pig IgG H&L (HRP)

Host species

Rabbit

Target species

Pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA

Clonality

Polyclonal

Format

F(ab')2

Pre-adsorbed

No

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine), 50% Tris buffered saline

Purification technique

Affinity purification Immunogen

Purification notes

ab136761 is purified by affinity chromatography and is thus particularly specific.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Preparation of Substrate Solutions:



- ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm


- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.


- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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