Suitable for Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA. Ideal for western blot.
Rabbit
Pig
IgG
Heavy & Light chains
HRP
Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA
Polyclonal
F(ab')2
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info 1/20 - 1/100 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info 1/20 - 1/100 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/100 - 1/1000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/20 - 1/100 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/500 - 1/5000 | Notes - |
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Suitable for Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA. Ideal for western blot.
Rabbit F(ab')2 Anti-Pig IgG H&L (HRP)
Rabbit
Pig
IgG
Heavy & Light chains
HRP
Flow Cyt, ICC, IHC-Fr, WB, IHC-P, ELISA
Polyclonal
F(ab')2
No
IgG
Liquid
Constituents: 50% Glycerol (glycerin, glycerine), 50% Tris buffered saline
Affinity purification Immunogen
ab136761 is purified by affinity chromatography and is thus particularly specific.
Blue Ice
+4°C
+4°C
Preparation of Substrate Solutions:
- ELISA: OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm
- Immunocytochemistry: DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
- Western Blot: 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.
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