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AB102345

Rabbit Anti-Goat IgG Fc (HRP) preadsorbed

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Suitable for IHC-P, ELISA, ICC, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission
Applications

ELISA, WB, IHC-P, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"<p>(Primary).</p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"<p>(Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000).</p>" } } }

Product details

By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

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