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AB175667

Rabbit Anti-Goat IgG H&L (Alexa Fluor® 405)

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(1 Publication)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 1 publication.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175667)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175667)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175667 Alexa Fluor® 405 Rabbit polyclonal Secondary Antibody to Goat IgG - H&L pre-adsorbed (blue) was then used at 1μg/ml for 1h as a tertiary antibody. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175667)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175667)

Key facts

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

IHC-P, ICC/IF, Flow Cyt, ELISA, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor<sup>®</sup> 405 dye (see difference below)<br>Ex max: Alexa Fluor<sup>®</sup> 405 = 402nm / DAPI = 359nm<br>Em max: Alexa Fluor<sup>®</sup> 405 = 421nm / DAPI = 461nm</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:12899-12911 PubMed35609329

2022

Curcumin alleviates rheumatoid arthritis progression through the phosphatidylinositol 3-kinase/protein kinase B pathway: an and study.

Applications

Unspecified application

Species

Unspecified reactive species

Zihan Xu,Wei Shang,Zhiming Zhao,Beibei Zhang,Chunli Liu,Hui Cai
View all publications

Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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