Rabbit Anti-Goat IgG H&L (Alexa Fluor® 555)
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(1 Publication)
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 1 publication.
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Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150142)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine / 10% normal rabbit serum in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150142 Alexa Fluor® 555 rabbit anti-goat IgG (H+L) (yellow) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
- Alexa Fluor®
Unknown
Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150142)
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Publications (1)
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Nature communications 14:4011 PubMed37419902
2023
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