JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB150142

Rabbit Anti-Goat IgG H&L (Alexa Fluor® 555)

Be the first to review this product! Submit a review

|

(1 Publication)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 1 publication.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150142)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150142)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine / 10% normal rabbit serum in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150142 Alexa Fluor® 555 rabbit anti-goat IgG (H+L) (yellow) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150142)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150142)

Key facts

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ELISA, IHC-Fr, Flow Cyt, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4011 PubMed37419902

2023

Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ao Fang,Yifan Wang,Naiyu Guan,Yanming Zuo,Lingmin Lin,Binjie Guo,Aisheng Mo,Yile Wu,Xurong Lin,Wanxiong Cai,Xiangfeng Chen,Jingjia Ye,Zeinab Abdelrahman,Xiaodan Li,Hanyu Zheng,Zhonghan Wu,Shuang Jin,Kan Xu,Yan Huang,Xiaosong Gu,Bin Yu,Xuhua Wang
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com