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AB175707

Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed

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(3 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175707)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175707)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175707, Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (yellow) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

The secondary antibody only control displays negative staining, as expected.

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175707)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (AB175707)

Key facts

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Goat IgG
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols 18:1379-1415 PubMed37020146

2023

Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish.

Applications

Unspecified application

Species

Unspecified reactive species

Kuan-Ting Huang,Jesse R Poganik,Saba Parvez,Sruthi Raja,Brian Miller,Marcus J C Long,Joseph R Fetcho,Yimon Aye

eLife 11: PubMed36300632

2022

Z-REX uncovers a bifurcation in function of Keap1 paralogs.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Van Hall-Beauvais,Jesse R Poganik,Kuan-Ting Huang,Saba Parvez,Yi Zhao,Hong-Yu Lin,Xuyu Liu,Marcus John Curtis Long,Yimon Aye

Molecular medicine (Cambridge, Mass.) 28:73 PubMed35764933

2022

FGF1 alleviates LPS-induced acute lung injury via suppression of inflammation and oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Qhaweni Dhlamini,Wei Wang,Guifeng Feng,Aiping Chen,Lei Chong,Xue Li,Quan Li,Jin Wu,Depu Zhou,Jie Wang,Hailin Zhang,Jin-San Zhang
View all publications
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Product promise

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