Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
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Publications
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- Nature protocols 18:1379-14152023Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuan-Ting Huang,Jesse R Poganik,Saba Parvez,Sruthi Raja,Brian Miller,Marcus J C Long,Joseph R Fetcho,Yimon AyePubMed 37020146
- eLife 11:2022Z-REX uncovers a bifurcation in function of Keap1 paralogs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Van Hall-Beauvais,Jesse R Poganik,Kuan-Ting Huang,Saba Parvez,Yi Zhao,Hong-Yu Lin,Xuyu Liu,Marcus John Curtis Long,Yimon AyePubMed 36300632
- Molecular medicine (Cambridge, Mass.) 28:732022FGF1 alleviates LPS-induced acute lung injury via suppression of inflammation and oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQhaweni Dhlamini,Wei Wang,Guifeng Feng,Aiping Chen,Lei Chong,Xue Li,Quan Li,Jin Wu,Depu Zhou,Jie Wang,Hailin Zhang,Jin-San ZhangPubMed 35764933
Key facts
- Host species
- Rabbit
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Horse, Chicken, Human, Pig
- Conjugation
- Alexa Fluor® 568
- Excitation/Emission
- Ex: 578nm, Em: 603nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
Key facts
- Description
- Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed
- Host species
- Rabbit
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Horse, Chicken, Human, Pig
- Conjugation
- Alexa Fluor® 568
- Excitation/Emission
- Ex: 578nm, Em: 603nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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2 product images
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175707)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175707, Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (yellow) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Alexa Fluor® 568 is pseudocolored in yellow.
The secondary antibody only control displays negative staining, as expected.
Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175707)
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