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AB150148

Rabbit Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed

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(1 Publication)

Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

3 Images
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150148)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150148)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150148 Alexa Fluor® 594 rabbit anti-goat IgG (H+L) (orange) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150148)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150148)

HeLa cells showing negative staining by ICC/IF using only tertiary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab150148 Alexa Fluor® 594 rabbit anti-goat IgG (H+L) (orange) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150148)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150148)

Key facts

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at an assay dependent dilution</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at an assay dependent dilution</p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Goat IgG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed36556214

2022

AXL and MET Tyrosine Kinase Receptors Co-Expression as a Potential Therapeutic Target in Malignant Pleural Mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Zito Marino,Carminia Maria Della Corte,Vincenza Ciaramella,Stefania Erra,Andrea Ronchi,Alfonso Fiorelli,Giovanni Vicidomini,Mario Santini,Giosuè Scognamiglio,Floriana Morgillo,Fortunato Ciardiello,Renato Franco,Marina Accardo
View all publications
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