Suitable for WB, ELISA, ICC, IHC-P, IHC-Fr.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/400 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/100 - 1/1000 | Notes - |
Application ICC | Reactivity Reacts | Dilution info 1/10 - 1/100 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/10 - 1/100 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info 1/10 - 1/100 | Notes - |
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Suitable for WB, ELISA, ICC, IHC-P, IHC-Fr.
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
ab136719 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity.
Preparation of Substrate Solutions:
ELISA: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH 5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
Immunocytochemistry: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH 6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
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