Rabbit anti-goat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot, dot blot and ELISA.
- Cited in over 210 publications
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- Nature communications 15:14962024Exosomes define a local and systemic communication network in healthy pancreas and pancreatic ductal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBárbara Adem,Nuno Bastos,Carolina F Ruivo,Sara Sousa-Alves,Carolina Dias,Patrícia F Vieira,Inês A Batista,Bruno Cavadas,Dieter Saur,José C Machado,Dawen Cai,Sonia A MeloPubMed 38383468
- eLife 12:2023Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiling Bian,Jiemin Zhu,Xiaobo Jia,Wenjun Liang,Sihan Yu,Zhiqiang Li,Wenxia Zhang,Yi RaoPubMed 38126335
- Biomaterials research 27:1242023Post-insertion technique to introduce targeting moieties in milk exosomes for targeted drug delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHochung Jang,Hyosuk Kim,Eun Hye Kim,Geonhee Han,Yeongji Jang,Yelee Kim,Jong Won Lee,Sang Chul Shin,Eunice EunKyeong Kim,Sun Hwa Kim,Yoosoo YangPubMed 38031117
- Nature communications 14:40482023The structural pathology for hypophosphatasia caused by malfunctional tissue non-specific alkaline phosphatase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYating Yu,Kewei Rong,Deqiang Yao,Qing Zhang,Xiankun Cao,Bing Rao,Ying Xia,Yi Lu,Yafeng Shen,Ying Yao,Hongtao Xu,Peixiang Ma,Yu Cao,An QinPubMed 37422472
- Journal of ovarian research 16:562023In-vivo oogenesis of oogonial and mesenchymal stem cells seeded in transplanted ovarian extracellular matrix.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeila Mirzaeian,Farideh Eivazkhani,Maryam Saber,Ashraf Moini,Fereshteh Esfandiari,Mojtaba Rezazadeh Valojerdi,Rouhollah FathiPubMed 36941728
- Frontiers in cell and developmental biology 11:11192202023Identification of protein candidates in spermatozoa of water buffalo () bulls helps in predicting their fertility status.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeema Karanwal,Ankit Pal,Jatinder Singh Chera,Vipul Batra,Arumugam Kumaresan,Tirtha K Datta,Rakesh KumarPubMed 36891514
- mBio 14:e03545222023Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandhya Ganesan,Natalie N Alvarez,Samuel Steiner,Karen M Fowler,Abigail K Corona,Craig R RoyPubMed 36728431
- Scientific reports 13:14832023ACSS2-mediated NF-κB activation promotes alkaliptosis in human pancreatic cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongwen Que,Feimei Kuang,Rui Kang,Daolin Tang,Jiao LiuPubMed 36707625
- Cells 12:2023Phosphorylation of IGFBP-3 by Casein Kinase 2 Blocks Its Interaction with Hyaluronan, Enabling HA-CD44 Signaling Leading to Increased NSCLC Cell Survival and Cisplatin Resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai-Ling Coleman,Michael Chiaramonti,Ben Haddad,Robert Ranzenberger,Heather Henning,Hind Al Khashali,Ravel Ray,Ban Darweesh,Jeffrey Guthrie,Deborah Heyl,Hedeel Guy EvansPubMed 36766747
- Nature communications 14:3782023Structural mechanism of BRD4-NUT and p300 bipartite interaction in propagating aberrant gene transcription in chromatin in NUT carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Yu,Yingying Liang,Claudia Kim,Anbalagan Jaganathan,Donglei Ji,Xinye Han,Xuelan Yang,Yanjie Jia,Ruirui Gu,Chunyu Wang,Qiang Zhang,Ka Lung Cheung,Ming-Ming Zhou,Lei ZengPubMed 36690674
Key facts
- Host species
- Rabbit
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ELISA, WB
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info 1/50000 - 1/100000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/5000 - 1/20000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Rabbit anti-goat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot, dot blot and ELISA.
- Cited in over 210 publications
Key facts
- Description
- Rabbit Anti-Goat IgG H&L (HRP)
- Host species
- Rabbit
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
Rabbit Anti-Goat IgG secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
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Full details and terms and conditions can be found here:
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7 product images
Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741)
ab6741 was used at dilution 1/5000 with the primary antibody Anti-TIM50 antibody ab23938 in WB. See the review on ab23938.
SecondaryAll lanes: Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741)
Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741)
Histone H3 antibody (ab12079) at 0.5 ug/ml, HeLa Cell Histone prep at 5 ug
Secondary
Rabbit polyclonal to Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 15kDAll lanes: Anti-Histone H3 antibody - ChIP Grade (ab12079) at 0.5 µg/mL
All lanes: HeLa Cell Histone prep at 5 µg
SecondaryAll lanes: Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Performed under reducing conditions.
Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741)
Secondary antibody - rabbit anti-goat HRP (ab6741)
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (Anti-beta Actin antibody - Loading Control ab8229) at 1/1000 dilution
Lane 1: HeLa whole cell (Human) at 20 µg
Lane 2: 3T3 cell (Mouse) at 20 µg
Lane 3: Rabbit Liver at 20 µg
Lane 4: MDCK cell (Dog) at 20 µg
Lane 5: EBTr cell (Cow) at 20 µg
Lane 6: SL-29 cell (Chicken) at 20 µg
Lane 7: CHO cell (Chinese Hamster) at 20 µg
SecondaryAll lanes: Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 42 kDa
Exposure time: 5min
Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741)
Western blot of EMSY on MCF-7 cell lysate.
Lane 1: ab4579 at 1/500
Lane 2: Anti-EMSY antibody ab123 at 1/500.
Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both Anti-EMSY antibody ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.
Secondary ab Lane 1: Rabbit polyclonal to Goat IgG H&L (HRP) ab6741 (1/5000)
Exposure time: 3 min.Secondary ab Lane 2: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090 (1/5000)
Exposure time: 10 sec.Lysate at 20μg/lane.
Lane 1 - Exposure time : 3 min.
Lane 2 - Exposure time : 10 sec.
Variability in the size at which EMSY runs by Western blot has been experLane 1: Anti-EMSY antibody (ab4579) at 1/500 dilution
Lane 2: Western blot - Anti-EMSY antibody (Anti-EMSY antibody ab123) at 1/500 dilution
All lanes: MCF-7 cell lysate at 20 µg
SecondaryLane 1: Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 141 kDa
Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741)
Western blot analysis of ab6741 used as a secondary antibody at 1/1000 dilution for 60 min at RT.
All lanes: Goat IgG at 100 ng
SecondaryAll lanes: Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/1000 dilution
ELISA - Rabbit Anti-Goat IgG H&L (HRP) (ab6741)
ELISA results of ab6741 against purified Goat IgG protein. Each well was coated in duplicate with 1.0 µg of Goat IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/22,700. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.
This image is courtesy of an anonymous customer review.ELISA - Rabbit Anti-Goat IgG H&L (HRP) (ab6741)
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