Rabbit Anti-Goat IgG H&L (HRP)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L (HRP) (AB97100)

IHC image of beta Actin staining in Human normal Colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8229, 10μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97100, 1/100 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

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Western blot - Rabbit Anti-Goat IgG H&L (HRP) (AB97100)

All lanes : Anti-beta Actin antibody (ab8229) at 1 μg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 μg per lane.
Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/5000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97100) at 1/20000 dilution

Developed using the ECL technique

Performed under reducing conditions.

Exposure time : 8 minutes

Secondary

All lanes:

Western blot - Rabbit Anti-Goat IgG H&L (HRP) (ab97100)

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