AB97105

Rabbit Anti-Goat IgG H&L (HRP) preadsorbed

Name: Rabbit Anti Goat (IgG) secondary antibody preadsorbed HRP (ab97105) | Abcam
Brand: Abcam Limited
SKU: AB97105
Price: 295 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(10 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 10 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Human, Pig

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

WB, ICC, IHC-P, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"ELISA-Primary" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"WB-Colorimetric 1:5,000 - 1:30,000; WB-Chemiluminescent 1:10,000 - 1:50,000" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2504:113-133 PubMed35467283

2022

Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance.

Applications

Unspecified application

Species

Unspecified reactive species

Marlene Reithmair,Anja Lindemann,Veronika Mussack,Michael W Pfaffl

PloS one 15:e0229606 PubMed32109241

2020

Postprandial transfer of colostral extracellular vesicles and their protein and miRNA cargo in neonatal calves.

Applications

Unspecified application

Species

Unspecified reactive species

Benedikt Kirchner,Dominik Buschmann,Vijay Paul,Michael W Pfaffl

Anesthesiology 132:107-120 PubMed31596735

2019

Propofol and Sevoflurane Differentially Impact MicroRNAs in Circulating Extracellular Vesicles during Colorectal Cancer Resection: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Dominik Buschmann,Florian Brandes,Anja Lindemann,Melanie Maerte,Petra Ganschow,Alexander Chouker,Gustav Schelling,Michael W Pfaffl,Marlene Reithmair

Journal of extracellular vesicles 8:1670935 PubMed31632620

2019

Transcriptomic profiling of cell-free and vesicular microRNAs from matched arterial and venous sera.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Hermann,Dominik Buschmann,Benedikt Kirchner,Melanie Borrmann,Florian Brandes,Stefan Kotschote,Michael Bonin,Anja Lindemann,Marlene Reithmair,Gustav Schelling,Michael W Pfaffl

Nucleic acids research 47:10212-10234 PubMed31538203

2019

HIF-transcribed p53 chaperones HIF-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Esha Madan,Taylor M Parker,Christopher J Pelham,Antonio M Palma,Maria L Peixoto,Masaki Nagane,Aliya Chandaria,Ana R Tomás,Rita Canas-Marques,Vanessa Henriques,Antonio Galzerano,Joaquim Cabral-Teixeira,Karuppaiyah Selvendiran,Periannan Kuppusamy,Carlos Carvalho,Antonio Beltran,Eduardo Moreno,Uttam K Pati,Rajan Gogna

Biomolecular detection and quantification 17:100089 PubMed31194192

2019

Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing-Enabling robust and non-invasive biomarker research.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Mussack,Georg Wittmann,Michael W Pfaffl

Cell proliferation 52:e12548 PubMed30507050

2018

Circ_0078767 suppresses non-small-cell lung cancer by protecting RASSF1A expression via sponging miR-330-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Chen,Zuozhang Yang,Chao Liu,Li Wang,Jun Yang,Long Chen,Wenhui Li

Journal of extracellular vesicles 7:1481321 PubMed29887978

2018

Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Dominik Buschmann,Benedikt Kirchner,Stefanie Hermann,Melanie Märte,Christine Wurmser,Florian Brandes,Stefan Kotschote,Michael Bonin,Ortrud K Steinlein,Michael W Pfaffl,Gustav Schelling,Marlene Reithmair

Clinical and vaccine immunology : CVI 23:451-9 PubMed27030590

2016

Novel Respiratory Syncytial Virus-Like Particle Vaccine Composed of the Postfusion and Prefusion Conformations of the F Glycoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Velasco Cimica,Hélène Boigard,Bipin Bhatia,John T Fallon,Alexandra Alimova,Paul Gottlieb,Jose M Galarza

Animal science journal = Nihon chikusan Gakkaiho 85:440-8 PubMed24450292

2014

Shotgun proteomic analysis of porcine colostrum and mature milk.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Ogawa,Takamitsu Tsukahara,Ryoichiro Nishibayashi,Masako Nakatani,Mie Okutani,Nobuo Nakanishi,Kazunari Ushida,Ryo Inoue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rabbit Anti-Goat IgG H&L (HRP) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (10)

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