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AB97101

Rabbit Anti-Goat IgG H&L preadsorbed

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Suitable for ICC, WB, ELISA, IHC-P. Preadsorbed to minimise non-specific binding and high background staining.
4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L preadsorbed (AB97101)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L preadsorbed (AB97101)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling alpha Tubulin acetyl k40 with ab289875 at 1/100 (9.83 μg/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab289875 for 30 mins at room temperature, followed by anti-goat IgG antibody (ab97101) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L preadsorbed (AB97101)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L preadsorbed (AB97101)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L preadsorbed (AB97101)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L preadsorbed (AB97101)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L preadsorbed (AB97101)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Goat IgG H&L preadsorbed (AB97101)

Key facts

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Horse, Chicken, Human, Pig

Pre-adsorbed

Yes

Applications

ELISA, ICC, WB, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/30000", "notes":"<p>with chemiluminescent detection.WB with Colorimetric detection: 1/1000 - 1/10000</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/10000", "notes":"<p>ELISA-Coating 1:100 - 1:500; ELISA-Primary 1:1000 - 1:10,000</p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Goat IgG
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Product promise

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