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AB6760

Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)

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(4 Publications)

Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 4 publications.

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

1 Images
Dot Blot - Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (AB6760)
  • Dot

Supplier Data

Dot Blot - Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (AB6760)

Dot blot analysis of ab6760.

All lanes:

Dot Blot - Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)

Lane 1:

Human IgG at 200 ng

Lane 2:

Human IgG at 66.67 ng

Lane 3:

Human IgG at 22.2 ng

Lane 4:

Human IgG at 7.4 ng

Lane 5:

Human IgG at 2.47 ng

Secondary

All lanes:

Rabbit anti-Human IgG Antibody Alkaline Phosphatase Conjugated at 1/1000 dilution

false

Key facts

Host species

Rabbit

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alkaline Phosphatase

Excitation/Emission
Applications

IHC-Fr, ICC/IF, IHC-P, WB, IM, ELISA, Dot

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Anti-HUMAN IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 8 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
See full target information IGHG1

Additional targets

IGHG4,IGHG3,IGHG2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:15817 PubMed29150665

2017

A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris.

Applications

WB

Species

Unspecified reactive species

Kate E Royle,Karen Polizzi

Journal of immunology (Baltimore, Md. : 1950) 190:5981-91 PubMed23690478

2013

Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rasmus Iversen,Roberto Di Niro,Jorunn Stamnaes,Knut E A Lundin,Patrick C Wilson,Ludvig M Sollid

Journal of neuropathology and experimental neurology 67:326-40 PubMed18379435

2008

Autoimmunity against myelin oligodendrocyte glycoprotein is dispensable for the initiation although essential for the progression of chronic encephalomyelitis in common marmosets.

Applications

ELISA

Species

Unspecified reactive species

S Anwar Jagessar,Paul A Smith,Erwin Blezer,Cecile Delarasse,Danielle Pham-Dinh,Jon D Laman,Jan Bauer,Sandra Amor,Bert 't Hart

Journal of immunology (Baltimore, Md. : 1950) 180:1326-37 PubMed18209026

2008

Fast progression of recombinant human myelin/oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis in marmosets is associated with the activation of MOG34-56-specific cytotoxic T cells.

Applications

ELISA

Species

Unspecified reactive species

Yolanda S Kap,Paul Smith,S Anwar Jagessar,Ed Remarque,Erwin Blezer,Gustav J Strijkers,Jon D Laman,Rogier Q Hintzen,Jan Bauer,Herbert P M Brok,Bert A 't Hart
View all publications
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