Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 4 publications.
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- Scientific reports 7:158172017A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris.
- Journal of immunology (Baltimore, Md. : 1950) 190:5981-912013Transglutaminase 2-specific autoantibodies in celiac disease target clustered, N-terminal epitopes not displayed on the surface of cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRasmus Iversen et. al.PubMed 23690478
- Journal of neuropathology and experimental neurology 67:326-402008Autoimmunity against myelin oligodendrocyte glycoprotein is dispensable for the initiation although essential for the progression of chronic encephalomyelitis in common marmosets.Applications:ELISAReactive species:Unspecified reactive speciesS Anwar Jagessar,Paul A Smith,Erwin Blezer,Cecile Delarasse,Danielle Pham-Dinh,Jon D Laman,Jan Bauer,Sandra Amor,Bert 't HartPubMed 18379435
- Journal of immunology (Baltimore, Md. : 1950) 180:1326-372008Fast progression of recombinant human myelin/oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis in marmosets is associated with the activation of MOG34-56-specific cytotoxic T cells.Applications:ELISAReactive species:Unspecified reactive speciesYolanda S Kap,Paul Smith,S Anwar Jagessar,Ed Remarque,Erwin Blezer,Gustav J Strijkers,Jon D Laman,Rogier Q Hintzen,Jan Bauer,Herbert P M Brok,Bert A 't HartPubMed 18209026
Key facts
- Host species
- Rabbit
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Expected | Dilution info - | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/200 - 1/1000 | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Additional Targets
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Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Recommended products
Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 4 publications.
Key facts
- Description
- Rabbit Anti-Human IgG H&L (Alkaline Phosphatase)
- Host species
- Rabbit
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 8
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride- Purification technique
- Affinity purification
- Purification notes
Anti-HUMAN IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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1 product image
Dot Blot - Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)
Dot blot analysis of ab6760.
All lanes: Dot Blot - Rabbit Anti-Human IgG H&L (Alkaline Phosphatase) (ab6760)
Lane 1: Human IgG at 200 ng
Lane 2: Human IgG at 66.67 ng
Lane 3: Human IgG at 22.2 ng
Lane 4: Human IgG at 7.4 ng
Lane 5: Human IgG at 2.47 ng
SecondaryAll lanes: Rabbit anti-Human IgG Antibody Alkaline Phosphatase Conjugated at 1/1000 dilution
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