Skip to main content

Suitable for IHC-Fr, ELISA, IM, Flow Cyt, ICC/IF. Cited in 2 publications.

Be the first to review this product! Submit a review

Publications

Key facts

Host species

Rabbit

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IHC-Fr, ELISA, IM, Flow Cyt, ICC/IF

Clonality

Polyclonal

Reactivity data

Application

IHC-Fr

Reactivity

Expected

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Expected

Dilution info

1/10000 - 1/50000

Notes

-

Application

IM

Reactivity

Expected

Dilution info

1/1000 - 1/5000

Notes

-

Application

Flow Cyt

Reactivity

Expected

Dilution info

1/500 - 1/2500

Notes

-

Application

ICC/IF

Reactivity

Expected

Dilution info

1/1000 - 1/5000

Notes

-

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

Targets

, ,

Alternative names

Recommended products

Suitable for IHC-Fr, ELISA, IM, Flow Cyt, ICC/IF. Cited in 2 publications.

Key facts

Description

Rabbit Anti-Human IgG H&L (FITC)

Host species

Rabbit

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IHC-Fr, ELISA, IM, Flow Cyt, ICC/IF

Clonality

Polyclonal

Pre-adsorbed

No

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.878% Sodium chloride, 0.424% Tripotassium orthophosphate

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Fluorochrome chart

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com