Rabbit anti-human IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot, dot blot and ELISA.
- Cited in over 110 publications
Images
Publications
Filter by
- Nature chemical biology 20:1012-10212024Vaccine design via antigen reorientation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuo Xu,Joshua J Carter,Chunfeng Li,Ashley Utz,Payton A B Weidenbacher,Shaogeng Tang,Mrinmoy Sanyal,Bali Pulendran,Christopher O Barnes,Peter S KimPubMed 38225471
- Cell reports. Medicine 4:1011562023Bioengineered human tissue regeneration and repair using endogenous stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiao Wei,Daniel T Baptista-Hon,Zi Wang,Gen Li,Tanja Herrler,Chuanchang Dai,Kai Liu,Baofu Yu,Xiaoxue Chen,Mei Yang,Dong Han,Yuanxu Gao,Ru-Lin Huang,Lifei Guo,Kang Zhang,Qingfeng LiPubMed 37586324
- Journal of gastroenterology 58:778-7892023Anti-integrin αvβ6 autoantibodies in patients with primary sclerosing cholangitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroyuki Yoshida,Masahiro Shiokawa,Takeshi Kuwada,Yuya Muramoto,Sakiko Ota,Yoshihiro Nishikawa,Hirona Maeda,Nobuyuki Kakiuchi,Kanako Okamoto,Hajime Yamazaki,Masataka Yokode,Takeharu Nakamura,Shimpei Matsumoto,Tomonori Hirano,Hirokazu Okada,Saiko Marui,Yuko Sogabe,Tomoaki Matsumori,Atsushi Mima,Norimitsu Uza,Yuji Eso,Atsushi Takai,Ken Takahashi,Yoshihide Ueda,Yuzo Kodama,Tsutomu Chiba,Hiroshi SenoPubMed 37310456
- BMC medicine 21:1832023Unexpectedly higher levels of anti-orthopoxvirus neutralizing antibodies are observed among gay men than general adult population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanmeng Feng,Yifan Zhang,Shengya Liu,Meng Guo,Haojie Huang,Cuiyuan Guo,Wanhai Wang,Wenhong Zhang,Heng Tang,Yanmin WanPubMed 37189197
- Experimental and therapeutic medicine 25:2642023PRMT5 inhibition ameliorates inflammation and promotes the osteogenic differentiation of LPS‑induced periodontal stem cells via STAT3/NF‑κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Zhang,Changshun Li,Jian Sun,Xiaobei TianPubMed 37206565
- Nature communications 14:21492023A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPayton A-B Weidenbacher,Mrinmoy Sanyal,Natalia Friedland,Shaogeng Tang,Prabhu S Arunachalam,Mengyun Hu,Ozan S Kumru,Mary Kate Morris,Jane Fontenot,Lisa Shirreff,Jonathan Do,Ya-Chen Cheng,Gayathri Vasudevan,Mark B Feinberg,Francois J Villinger,Carl Hanson,Sangeeta B Joshi,David B Volkin,Bali Pulendran,Peter S KimPubMed 37069151
- European journal of medical research 28:1282023The characters of antibodies against PLA2R in healthy individuals and in the patient with PLA2R associated membranous nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Jiao Cheng,Miao Wang,Jia Wang,Zhao Cui,Ming-Hui ZhaoPubMed 36935517
- Experimental and therapeutic medicine 25:1772023ACSM3 suppresses proliferation and induces apoptosis and cell cycle arrest in acute myeloid leukemia cells via the regulation of IGF2BP2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Zheng,Jinjun Wu,Linlan Song,Bo HuangPubMed 37006876
- Arthritis research & therapy 25:252023Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkio Umemoto,Takeshi Kuwada,Koichi Murata,Masahiro Shiokawa,Sakiko Ota,Yoshiki Murotani,Akihiro Itamoto,Kohei Nishitani,Hiroyuki Yoshitomi,Takayuki Fujii,Akira Onishi,Hideo Onizawa,Kosaku Murakami,Masao Tanaka,Hiromu Ito,Hiroshi Seno,Akio Morinobu,Shuichi MatsudaPubMed 36804906
- Experimental and therapeutic medicine 25:1542023KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Yang,Qiaorong Zhang,Ziyuan Yang,Jiaming Shu,Lingling Zhang,Yangyang Yao,Xiaolang Wang,Xianxian LiuPubMed 36911368
Key facts
- Host species
- Rabbit
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- cELISA, Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info 1/5000 - 1/25000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/130000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Additional Targets
, ,
Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Recommended products
Rabbit anti-human IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot, dot blot and ELISA.
- Cited in over 110 publications
Key facts
- Description
- Rabbit Anti-Human IgG H&L (HRP)
- Host species
- Rabbit
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- cELISA, Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)
ELISA results of ab6759 tested against purified human IgG protein.Each well was coated in duplicate with 1.0 μg of human IgG. The starting dilution of antibody was 5 μg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
Dot Blot - Rabbit Anti-Human IgG H&L (HRP) (ab6759)
Dot blot analysis of ab6759.
All lanes: Dot Blot - Rabbit Anti-Human IgG H&L (HRP) (ab6759)
Lane 1: Human IgG at 100 ng
Lane 2: Human IgG at 33.3 ng
Lane 3: Human IgG at 11.1 ng
Lane 4: Human IgG at 3.7 ng
Lane 5: Human IgG at 1.23 ng
SecondaryAll lanes: Rabbit Anti-Human IgG Peroxidase Conjugated Antibody at 1/1000 dilution
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com