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AB288280

Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free

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Rabbit Anti-Human IgG Fc is an unconjugated secondary antibody that is suitable for ELISA.

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

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ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free (AB288280)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free (AB288280)

This data was developed using ab125909, the same antibody clone in a different buffer formulation.

Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free (AB288280)
  • ELISA

Lab

ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free (AB288280)

This data was developed using ab125909, the same antibody clone in a different buffer formulation.

Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free (AB288280)
  • ELISA

Lab

ELISA - Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc - BSA and Azide free (AB288280)

This data was developed using ab125909, the same antibody clone in a different buffer formulation.

Direct ELISA antibody dose-response curve using ab125909 between 0-1000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Unconjugated

    Rabbit monoclonal [H169-1-5] Anti-Human IgG Fc

Key facts

Host species

Rabbit

Target species

Human

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Applications

ELISA

applications

Clonality

Monoclonal

Clone number

H169-1-5

Isotype

IgG

Specificity

ab125909 is an IgG Fc specific antibody. The antibody recognizes HIgG, IgG1 kappa, IgG1 lambda, IgG2 kappa, IgG2 lambda, IgG3 kappa, IgG3 lambda, IgG4 kappa and IgG4 lambda Fc. ab125909 does not recognize HIgA, HIgM or F(ab)2.

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Reactivity data

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Product details

ab288280 is the carrier-free version of ab125909.

This antibody reacts with Fc region of Human IgG.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
See full target information IGHG1
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Product promise

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