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Recombinant secondary antibody suitable for ELISA, WB. Cited in 3 publications.

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Images

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919), expandable thumbnail
  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919), expandable thumbnail
  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919), expandable thumbnail
  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919), expandable thumbnail
  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919), expandable thumbnail

Publications

Key facts

Host species
Rabbit
Target species
Human
Target isotype
kappa
Target specificity
Light chain
Applications
ELISA, WB
Clonality
Monoclonal

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Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
1 µg/mL
Notes

For unpurified use at 1/1000 - 1/10000.

Application
WB
Reactivity
Reacts
Dilution info
1/5000
Notes

For unpurified use at 1/1000 - 1/10000.

Target data

Function

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

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Recombinant secondary antibody suitable for ELISA, WB. Cited in 3 publications.

Key facts

Description
Rabbit monoclonal [H19-5] Anti-Human kappa light chain
Host species
Rabbit
Target species
Human
Target isotype
kappa
Target specificity
Light chain
Applications
ELISA, WB
Clonality
Monoclonal
Clone number
H19-5
Pre-adsorbed
No
Specificity

ab125919 is a IgG kappa light chain Fab’2 specific antibody. The antibody recognises Human IgG, IgG1 kappa, IgG2 kappa, IgG3 kappa, IgG4 kappa, IgA, IgM and F(ab)2. ab125919 does not recognise IgG1 lambda, IgG2 lambda, IgG3 lambda, IgG4 lambda or Fc.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Purification technique
Affinity purification Protein A

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919), expandable thumbnail

    Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    Blocking and diluting buffer: 5% NFDM/TBST.

    Lane 1: Human spleen tissue lysate at 20 µg

    Lane 2: Human thymus tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Lanes 1 - 2: Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution

  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919), expandable thumbnail

    Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Human tonsil tissue lysate at 20 µg

    Secondary

    Lane 1: Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution

    Lane 1: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919), expandable thumbnail

    ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919), expandable thumbnail

    ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919), expandable thumbnail

    Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    This data was developed using ab125919, the same antibody clone in a different buffer formulation.

    Lane 1: Human spleen tissue lysate

    Lane 2: Human thymus tissue lysate

    Lane 3: Human tonsil tissue lysate

    Secondary

    All lanes: Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

  • ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919), expandable thumbnail

    ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

    ELISA dose-responsive curve using purified ab125919 at a range of concentrations.

    Antigen concentation: 1000ng/ml.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), 1/2500.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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