AB125919

Rabbit monoclonal [H19-5] Anti-Human kappa light chain

RabMAb
Recombinant
What is this?

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(4 Publications)

Recombinant secondary antibody suitable for ELISA, WB. Cited in 4 publications.

View Alternative Names

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

6 Images

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Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

This data was developed using ab125919, the same antibody clone in a different buffer formulation.

Lane 1:

Human spleen tissue lysate

Lane 2:

Human thymus tissue lysate

Lane 3:

Human tonsil tissue lysate

Secondary

All lanes:

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919)

false

Unknown

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

Blocking and diluting buffer : 5% NFDM/TBST.

Lane 1:

Human spleen tissue lysate at 20 µg

Lane 2:

Human thymus tissue lysate at 20 µg

Secondary

Lanes 1 - 2:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Lanes 1 - 2:

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution

false

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

ELISA

Supplier Data

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA

Lab

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

ELISA

Unknown

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

ELISA dose-responsive curve using purified ab125919 at a range of concentrations.

Antigen concentation : 1000ng/ml.

Secondary antibody : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), 1/2500.

Unknown

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Human tonsil tissue lysate at 20 µg

Secondary

Lane 1:

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (ab125919) at 1/5000 dilution

Lane 1:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

false

Carrier free

Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free
665 Alexa Fluor® 647

Rabbit monoclonal [H19-5] Anti-Human kappa light chain (Alexa Fluor® 647)

Key facts

Host species

Rabbit

Target species

Human

Target isotype

kappa

Target specificity

Light chain

Minimal cross-reactivity

Pre-adsorbed

Applications

WB, ELISA

applications

Clonality

Monoclonal

Clone number

H19-5

Isotype

IgG

Specificity

ab125919 is a IgG kappa light chain Fab’2 specific antibody. The antibody recognises Human IgG, IgG1 kappa, IgG2 kappa, IgG3 kappa, IgG4 kappa, IgA, IgM and F(ab)2. ab125919 does not recognise IgG1 lambda, IgG2 lambda, IgG3 lambda, IgG4 lambda or Fc.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg/mL", "notes":"For unpurified use at 1/1000 - 1/10000." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000", "notes":"For unpurified use at 1/1000 - 1/10000." } } }

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Product details

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKC

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 11:1346259 PubMed38756530

2024

The dispensability of V-V pairing and the indispensability of V domain integrity in the IgG1 secretion process.

Applications

Unspecified application

Species

Unspecified reactive species

Juho Choi,Yerin Jeon,Youngin Roh,Jeongyun Jang,Eunbin Lee,Luigie Villamante,Minjae Kim,Myung-Hee Kwon

Nature communications 14:3897 PubMed37400446

2023

A rapid cell-free expression and screening platform for antibody discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew C Hunt,Bastian Vögeli,Ahmed O Hassan,Laura Guerrero,Weston Kightlinger,Danielle J Yoesep,Antje Krüger,Madison DeWinter,Michael S Diamond,Ashty S Karim,Michael C Jewett

Journal of orthopaedic surgery and research 17:246 PubMed35459188

2022

microRNA-4701-5p protects against interleukin-1β induced human chondrocyte CHON-001 cells injury via modulating HMGA1.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Cheng Chen,Jie Song

Scientific reports 10:2140 PubMed32034177

2020

Assembly and Folding Properties of Cytosolic IgG Intrabodies.

Applications

Unspecified application

Species

Unspecified reactive species

Youngsil Seo,Yeonjin Lee,Minjae Kim,Hyunjoon Park,Myung-Hee Kwon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit monoclonal [H19-5] Anti-Human kappa light chain

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

Related conjugates and formulations (2)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Applications

Clonality

Clone number

Isotype

Specificity

Complementary Products

Primary Antibodies

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise