Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free
- RabMAb
- Recombinant
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- ELISA
Supplier Data
ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)
This data was developed using ab125919, the same antibody clone in a different buffer formulation.
Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
- ELISA
Lab
ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)
This data was developed using ab125919, the same antibody clone in a different buffer formulation.
Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
- ELISA
Unknown
ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)
This data was developed using ab125919, the same antibody clone in a different buffer formulation.
ELISA dose-responsive curve using purified ab125919 at a range of concentrations.
Antigen concentation : 1000ng/ml.
Secondary antibody : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), 1/2500.
Reactivity data
Product details
ab288283 is the carrier-free version of ab125919
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com