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AB288283

Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free

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Recombinant secondary antibody suitable for ELISA, WB.
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ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)

This data was developed using ab125919, the same antibody clone in a different buffer formulation.

Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)
  • ELISA

Lab

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)

This data was developed using ab125919, the same antibody clone in a different buffer formulation.

Direct ELISA antibody dose-response curve using ab125919 between 0-2500 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)
  • ELISA

Unknown

ELISA - Rabbit monoclonal [H19-5] Anti-Human kappa light chain - BSA and Azide free (AB288283)

This data was developed using ab125919, the same antibody clone in a different buffer formulation.

ELISA dose-responsive curve using purified ab125919 at a range of concentrations.

Antigen concentation : 1000ng/ml.

Secondary antibody : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), 1/2500.

Key facts

Host species

Rabbit

Target species

Human

Target isotype

kappa

Target specificity

Light chain

Minimal cross-reactivity
Pre-adsorbed

No

Applications

ELISA, WB

applications

Clonality

Monoclonal

Clone number

H19-5

Isotype

IgG

Specificity

ab125919 is a IgG kappa light chain Fab’2 specific antibody. The antibody recognises Human IgG, IgG1 kappa, IgG2 kappa, IgG3 kappa, IgG4 kappa, IgA, IgM and F(ab)2. ab125919 does not recognise IgG1 lambda, IgG2 lambda, IgG3 lambda, IgG4 lambda or Fc.

Reactivity data

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Product details

ab288283 is the carrier-free version of ab125919

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

Target data

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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