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AB239415

Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 - BSA and Azide free

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Recombinant secondary antibody suitable for ELISA.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

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ELISA - Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 - BSA and Azide free (AB239415)
  • ELISA

Lab

ELISA - Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3 - BSA and Azide free (AB239415)

Direct ELISA antibody dose-response curve using ab125904 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab125904).

  • Unconjugated

    Rabbit monoclonal [M111-2] Anti-Mouse IgG1, IgG2a, IgG3

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG1, IgG2a, IgG3

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Applications

ELISA

applications

Clonality

Monoclonal

Clone number

M111-2

Isotype

IgG

Specificity

Recognizes Mouse IgG1, IgG2a and IgG3 and Rat IgG.

Reactivity data

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Product details

ab239415 is the carrier-free version of ab125904.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Human: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Product protocols

Target data

See full target information Ighg1

Additional targets

Ighg1,,undefined

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com