Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling SHP2 with ab290646 at 1/500 (2.018 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining in human liver (PMID : 8216283).The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling p53 with ab308609 at 1/100 (10.28 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human cardiac muscle. The section was incubated with ab308609 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's tissue labeling Bcl6 with ab307681 at 1/1000 (0.992 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human non-Hodgkin's B-cell lymphoma. The section was incubated with ab307681 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling SHP2 with ab290646 at 1/500 (2.018 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in human ovarian cancer.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Synaptophysin with ab309493 at 1/20000 (0.047 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Low expression tissue : almost no staining on human kidney. The section was incubated with ab309493 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Synaptophysin with ab309493 at 1/20000 (0.047 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the islet in human pancreas. The section was incubated with ab309493 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling CD3 epsilon with ab313799 at 1/100 (9.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human cerebrum. The section was incubated with ab313799 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissues labeling MUC1 with ab70475 at 1/5000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab70475 for 30 mins at room temperature and followed by mouse IgG antibody (ab125913, 1 : 1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissues labeling MUC1 with ab70475 at 1/5000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab70475 for 30 mins at room temperature and followed by mouse IgG antibody (ab125913, 1 : 1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded cells labeling p53 with ab308609 at 1/100 (10.28 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly nuclear staining on (A) wild-type HAP1 cell pellet, no staining on (B) p53 knockout HAP1 cell pellet. The section was incubated with ab308609 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue labeling p53 with ab308609 at 1/100 (10.28 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Mainly nuclear staining on human endometrial cancer. The section was incubated with ab308609 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Nuclear staining in human breast cancer. The section was heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was incubated with ab52484 at 1 : 20000 (0.053 μg/ml) for 10 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling ECT2 with ab324657 at 1/100 (9.37 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human colon cancer.
The primary antibody was incubated for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling ECT2 with ab324657 at 1/100 (9.37 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cervical cancer.
The primary antibody was incubated for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD3 epsilon with ab313799 at 1/100 (9.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab313799 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ECT2 with ab324657 at 1/100 (9.37 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human pancreas.
The primary antibody was incubated for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl6 with ab307681 at 1/1000 (0.992 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human tonsil. The section was incubated with ab307681 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Cytoplasmic staining on rat cerebrum.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CD3 epsilon with ab313799 at 1/100 (9.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse cerebrum. The section was incubated with ab313799 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling CD3 epsilon with ab313799 at 1/100 (9.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat cerebrum. The section was incubated with ab313799 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Synaptophysin with ab309493 at 1/20000 (0.047 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab309493 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Synaptophysin with ab309493 at 1/20000 (0.047 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with ab309493 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling SHP2 with ab290646 at 1/2000 (0.505 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in rat kidney.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling SHP2 with ab290646 at 1/2000 (0.505 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in mouse kidney.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3 epsilon with ab313799 at 1/100 (9.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse spleen. The section was incubated with ab313799 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD3 epsilon with ab313799 at 1/100 (9.09 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat spleen. The section was incubated with ab313799 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling SHP2 with ab290646 at 1/2000 (0.505 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in rat cerebrum.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling SHP2 with ab290646 at 1/2000 (0.505 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining in mouse cerebrum.The section was incubated with ab290646 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Nuclear staining in rat lung. The section was heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was incubated with ab52484 (1 : 50000 (0.021 μg/ml)) for 10 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Nuclear staining in mouse lung. The section was heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was incubated with ab52484 (1 : 50000 (0.021 μg/ml)) for 10 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Rat hippocampus labeling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus labeling Glutamate Receptor 1 (AMPA subtype) with ab174785 at 1/10000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on rat cardiac muscle.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ELISA

Lab

ELISA - Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (AB125913)

Direct ELISA antibody dose-response curve using ab125913 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.