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AB125916

Rabbit monoclonal [MIgG-R180-9-8] Anti-Mouse IgG1+IgG2 H&L

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Recombinant secondary antibody suitable for ELISA.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

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ELISA - Rabbit monoclonal [MIgG-R180-9-8] Anti-Mouse IgG1+IgG2 H&L (AB125916)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [MIgG-R180-9-8] Anti-Mouse IgG1+IgG2 H&L (AB125916)

Direct ELISA antibody dose-response curve using ab125916 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG1, IgG2

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Applications

ELISA

applications

Clonality

Monoclonal

Clone number

MIgG-R180-9-8

Isotype

IgG

Specificity

ab125916 is specific for Mouse IgG1 and Mouse IgG2a. The antibody does not recognise Mouse IgG2b, Mouse IgG3, Mouse IgA, Mouse IgM, Human IgG or Rat IgG.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000", "notes":"<p></p>" } } }
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Product details

ab125916 has weak cross-reactivity with mouse IgG1.

Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Secondary Antibodies

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com