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AB238623

Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin)

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Suitable for ELISA, WB.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

3 Images
ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623)

ELISA of mouse immunoglobulins shows ab238623 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623)

A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Western blot - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623)
  • WB

Supplier Data

Western blot - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623)

This antibody reacts to non-reduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).

Lane 1:

Non-reduced (-) mouse IgG1, 20 ng/lane

Lane 2:

Reduced (+) mouse IgG1, 20 ng/lane

Secondary

All lanes:

Western blot - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623) at 0.2 µg/mL

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Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG1

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

WB, ELISA

applications

Clonality

Monoclonal

Clone number

RM106

Isotype

IgG

Immunogen

Full Length Protein corresponding to Mouse Ighg1. The exact immunogen used to generate this antibody is proprietary information.

P01868

Specificity

ab238623 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

The Fc region of ab238623 has been engineered to eliminate Fc receptor binding.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.005-2 µg/mL", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.1-5 µg/mL", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Product promise

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