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Suitable for ELISA, WB.

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Images

Western blot - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623), expandable thumbnail
  • ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623), expandable thumbnail
  • ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623), expandable thumbnail

Key facts

Host species
Rabbit
Target species
Mouse
Target isotype
IgG1
Target specificity
Fc region
Conjugation
Biotin
Applications
ELISA, WB
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information. Database link P01868

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
0.00500-2.00000 µg/mL
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
0.10000-5.00000 µg/mL
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Additional Targets

Alternative names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Recommended products

Suitable for ELISA, WB.

Key facts

Description
Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin)
Host species
Rabbit
Target species
Mouse
Target isotype
IgG1
Target specificity
Fc region
Conjugation
Biotin
Applications
ELISA, WB
Clonality
Monoclonal
Clone number
RM106
Pre-adsorbed
No
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information. Database link P01868
Specificity

ab238623 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

The Fc region of ab238623 has been engineered to eliminate Fc receptor binding.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Protein A
Purification notes

Purified from an animal origin–free culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623), expandable thumbnail

    Western blot - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)

    This antibody reacts to non-reduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).

    Lane 1: Non-reduced (-) mouse IgG1, 20 ng/lane

    Lane 2: Reduced (+) mouse IgG1, 20 ng/lane

    Secondary

    All lanes: Western blot - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623) at 0.2 µg/mL

  • ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623), expandable thumbnail

    ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)

    A titer ELISA of mouse IgG1. The plate was coated with different amount of mouse IgG1. A serial dilution of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623), expandable thumbnail

    ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (ab238623)

    ELISA of mouse immunoglobulins shows ab238623 reacts to the Fc region of mouse IgG1; no cross reactivity with IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238623 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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