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AB238532

Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin)

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Recombinant secondary antibody suitable for ELISA, WB.
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ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)

A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)

ELISA of mouse immunoglobulins shows ab238532 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Western blot - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)
  • WB

Supplier Data

Western blot - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)

Lane 1:

Non-reduced mouse IgG2b at 20 µg

Lane 2:

Reduced mouse IgG2b at 20 µg

Secondary

All lanes:

Western blot - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532) at 0.2 µg/mL

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Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG2b

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

WB, ELISA

applications

Clonality

Monoclonal

Clone number

RM108

Isotype

IgG

Specificity

ab238532 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM108 has been engineered to eliminate Fc receptor binding.

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Secondary Antibodies

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Secondary Antibodies

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Primary Antibodies

AB190482

Anti-Mouse IgG2b antibody [RM108]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse IgG2b antibody [RM108] (AB190482)

  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.005-0.2 µg/mL", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.1-0.5 µg/mL", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab238532 was purified from an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

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Target data

See full target information Mouse IgG2b
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Product promise

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