Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (AB238617)

Paraffin-embedded human lymphoid tissue stained for IgG4 heavy chain using ab238617 at 10 μg/mL in immunohistochemical analysis.

• ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (AB238617)

A titer ELISA using ab238617. The plate was coated with different amounts of human IgG4. A serial dilution of ab238617 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.``

• sELISA

Supplier Data

Sandwich ELISA - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (AB238617)

Sandwich ELISA using ab238617 as the capture antibody, and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.``

• sELISA

Supplier Data

Sandwich ELISA - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (AB238617)

Sandwich ELISA using ab238617 as the capture antibody at 200ng/well, and a Biotinylated anti-human light chains (κ+ λ) antibody as the detection antibody, followed by an AP conjugated streptavidin.``

• WB

Supplier Data

Western blot - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (AB238617)

Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms (Lane 4). No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.

Lane 1:

Non-reduced (-) and reduced (+) forms of Human IgG1

Lane 2:

Non-reduced (-) and reduced (+) forms of Human IgG2

Lane 3:

Non-reduced (-) and reduced (+) forms of Human IgG3

Lane 4:

Non-reduced (-) and reduced (+) forms of Human IgG4

Lane 5:

Non-reduced (-) and reduced (+) forms of Mouse IgG

Lane 6:

Non-reduced (-) and reduced (+) forms of Rat IgG

Lane 7:

Non-reduced (-) and reduced (+) forms of Goat IgG

Secondary

All lanes:

Western blot - Rabbit monoclonal [RM120] Anti-Human IgG4 heavy chain (Biotin) (ab238617) at 2 µg/mL

false