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AB272185

Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin)

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Recombinant secondary antibody suitable for ELISA.

View Alternative Names

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

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ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (AB272185)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (AB272185)

ELISA shows that ab272185 specifically reacts to human IgG4 and does not cross react with human IgG1, IgG2, IgG3.

ab272185 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

50ng/well to 200ng/well was used for capture, and 0.05ug/mL to 0.2ug/mL was used for detection.

Sandwich ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (AB272185)
  • sELISA

Supplier Data

Sandwich ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (AB272185)

Sandwich ELISA using ab272185 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.

Sandwich ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (AB272185)
  • sELISA

Supplier Data

Sandwich ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (AB272185)

Sandwich ELISA using ab272185 as the capture antibody, and a Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an AP conjugated streptavidin.

ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (AB272185)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM217] Anti-Human IgG4 Fc (Biotin) (AB272185)

A titer ELISA analysis using ab272185. The plate was coated with different amounts of human IgG4. A serial dilution of ab272185 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Key facts

Host species

Rabbit

Target species

Human

Target isotype

IgG4

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

ELISA

applications

Clonality

Monoclonal

Clone number

RM217

Isotype

IgG4

Immunogen

Synthetic Peptide within Human IGHG4. The exact immunogen used to generate this antibody is proprietary information.

P01861

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>50ng/well – 200ng/well (for Capture)<br>0.05ug/mL – 0.2ug/mL (for Detection)</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
From an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
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Product protocols

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Target data

The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection. This supplementary information is collated from multiple sources and compiled automatically.
See full target information IGHG4
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