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AB238619

Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin)

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Suitable for ICC, ELISA, WB.
3 Images
Western blot - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619)
  • WB

Supplier Data

Western blot - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619)

This antibody reacts to non-reduced IgG2c.

Lane 1:

Non-reduced (-) mouse IgG2c

Lane 2:

Reduced (+) mouse IgG2c

Secondary

All lanes:

Western blot - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619) at 0.5 µg/mL

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ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619)

ELISA of IgG from BalB/C and C57BL6 shows ab238619 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab2308619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619)

A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of ab238619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG2c

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

ICC, WB, ELISA

applications

Clonality

Monoclonal

Clone number

RM223

Isotype

IgG

Specificity

ab238619 reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.5-2 µg/mL", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.01-0.2 µg/mL", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.5-2 µg/mL", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

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Target data

See full target information Mouse IgG2c
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