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Suitable for ICC, ELISA, WB.

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Images

Western blot - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619), expandable thumbnail
  • ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619), expandable thumbnail
  • ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (AB238619), expandable thumbnail

Key facts

Host species
Rabbit
Target species
Mouse
Target isotype
IgG2c
Target specificity
Fc region
Conjugation
Biotin
Applications
ICC, ELISA, WB
Clonality
Monoclonal

Reactivity data

Application
ICC
Reactivity
Reacts
Dilution info
0.50000-2.00000 µg/mL
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
0.01000-0.20000 µg/mL
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
0.50000-2.00000 µg/mL
Notes

-

Target data

Recommended products

Suitable for ICC, ELISA, WB.

Key facts

Description
Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin)
Host species
Rabbit
Target species
Mouse
Target isotype
IgG2c
Target specificity
Fc region
Conjugation
Biotin
Applications
ICC, ELISA, WB
Clonality
Monoclonal
Clone number
RM223
Pre-adsorbed
No
Specificity

ab238619 reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Protein A
Purification notes

Purified from an animal origin–free culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619), expandable thumbnail

    Western blot - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619)

    This antibody reacts to non-reduced IgG2c.

    Lane 1: Non-reduced (-) mouse IgG2c

    Lane 2: Reduced (+) mouse IgG2c

    Secondary

    All lanes: Western blot - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619) at 0.5 µg/mL

  • ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619), expandable thumbnail

    ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619)

    A titer ELISA of mouse IgG2c. The plate was coated with different amounts of mouse IgG2c. A serial dilution of ab238619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619), expandable thumbnail

    ELISA - Rabbit monoclonal [RM223] Anti-Mouse IgG2c Fc (Biotin) (ab238619)

    ELISA of IgG from BalB/C and C57BL6 shows ab238619 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab2308619 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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