AB150125

Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed

Name: Rabbit Anti-Mouse IgG (Alexa Fluor® 488) preadsorbed secondary antibody (ab150125) | Abcam
Brand: Abcam Limited
SKU: AB150125

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(16 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 16 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

3 Images

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150125)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150125)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 10μg/ml) overnight at +4°C. The secondary antibody (green) was ab150125 Alexa Fluor® 488 rabbit anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150125)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (green) was ab150125 Alexa Fluor® 488 rabbit anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150125)

Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150125)

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

Flow Cyt, IHC-Fr, ICC/IF, ELISA, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody." }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:531 PubMed40195555

2025

A structural and mechanistic model for BSEP dysfunction in PFIC2 cholestatic disease.

Applications

Unspecified application

Species

Unspecified reactive species

Clémence Gruget,Bharat G Reddy,Jonathan M Moore

Cell reports 43:114594 PubMed39116203

2024

RTEL1 helicase counteracts RAD51-mediated homologous recombination and fork reversal to safeguard replicating genomes.

Applications

Unspecified application

Species

Unspecified reactive species

Suruchi Dixit,Tarun Nagraj,Debanjali Bhattacharya,Sneha Saxena,Satyaranjan Sahoo,Rajani Kant Chittela,Kumar Somyajit,Ganesh Nagaraju

Acta biochimica et biophysica Sinica 56:730-739 PubMed38655617

2024

Bronchial thermoplasty decreases airway remodeling by inhibiting autophagy via the AMPK/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Peng Fu,Wenting Huang,Liang Long,Fa Long,Shengming Liu

Royal Society open science 10:231023 PubMed37859832

2023

Mechanical loading due to muscle movement regulates establishment of the collagen network in the developing murine skeleton.

Applications

Unspecified application

Species

Unspecified reactive species

Saima Ahmed,Andrew V Rogers,Niamh C Nowlan

Bioactive materials 31:408-421 PubMed37692912

2023

Combination of biodegradable hydrogel and antioxidant bioadhesive for treatment of breast cancer recurrence and radiation skin injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuodan Zhang,Qiannan Cao,Yi Xia,Chunyan Cui,Ying Qi,Qian Zhang,Yuanhao Wu,Jianfeng Liu,Wenguang Liu

International journal of molecular sciences 24: PubMed37240083

2023

AEC and AFMSC Transplantation Preserves Fertility of Experimentally Induced Rat Varicocele by Expressing Differential Regenerative Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Peserico,Barbara Barboni,Valentina Russo,Delia Nardinocchi,Maura Turriani,Costanza Cimini,Nicola Bernabò,Ornella Parolini,Antonietta Rosa Silini,Ivana Antonucci,Liborio Stuppia,Paolo Berardinelli,Ilaria Falanga,Davide Perruzza,Luca Valbonetti,Annunziata Mauro

Aging 15:2046-2065 PubMed36988497

2023

Baixiangdan capsule and Shuyu capsule regulate anger-out and anger-in, respectively: GB1-mediated GABA can regulate 5-HT levels in multiple brain regions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoju Liu,Haijuan Wang,Xiaoyu Wang,Yinxia Ning,Wei Liu,Jie Gao

Molecular medicine reports 27: PubMed36799156

2023

P‑hydroxybenzyl alcohol ameliorates neuronal cerebral ischemia‑reperfusion injury by activating mitochondrial autophagy through SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglin Yu,Yuan Luo,Liping Yang,Pu Chen,Xiaohua Duan

International journal of medical sciences 19:596-608 PubMed35370459

2022

Loss-of-function Mutations K11E or E271K Lead to Novel Tumor Suppression, Implicate Nucleolar Helicase DDX24 Oncogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglin Li,Xiaoyun Chen,Jiebing Gao,Jianzhong Xian,Zhijun Li,Lei Bi,Min Yang,Shuai Yang,Hongjun Jin,Hong Shan

Viruses 12: PubMed32604892

2020

HHV-6 Infection and Chemokine RANTES Signaling Pathway Disturbance in Patients with Autoimmune Thyroiditis.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Sultanova,Maksims Cistjakovs,Liba Sokolovska,Katerina Todorova,Egils Cunskis,Modra Murovska

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150125)

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150125)

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150125)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (16)

Product promise