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Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.


Images

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (AB150126), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (AB150126), expandable thumbnail
  • Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (AB150126), expandable thumbnail

Publications

Key facts

Host species
Rabbit
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Rat, Human
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Applications
ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
Clonality
Polyclonal

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/2000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/200.00000 - 1/1082.00000
Notes

-

Additional Targets

Alternative names

Recommended products

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

Key facts

Description
Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed
Host species
Rabbit
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Rat, Human
Conjugation
Alexa Fluor® 555
Excitation/Emission
Ex: 555nm, Em: 565nm
Applications
ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Immunogen
Purification notes

Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150126), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150126)

    ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150126 Alexa Fluor® 555 rabbit anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150126), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150126)

    HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150126 Alexa Fluor® 555 rabbit anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150126), expandable thumbnail

    Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (ab150126)

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Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com