AB175703

Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed

Name: Rabbit Anti Mouse (IgG) secondary antibody preadsorbed Alexa Fluor® 568
Brand: Abcam Limited
SKU: AB175703
Price: 300 USD
Availability: InStock

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(5 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175703)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175703)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175703 Alexa Fluor® 568 rabbit anti-mouse IgG (H+L) preadsorbed used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175703)

Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175703)

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

ELISA, IHC-P, ICC/IF, IHC-Fr, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:8547563 PubMed35799894

2022

CXCR4/CX43 Regulate Diabetic Neuropathic Pain via Intercellular Interactions between Activated Neurons and Dysfunctional Astrocytes during Late Phase of Diabetes in Rats and the Effects of Antioxidant N-Acetyl-L-Cysteine.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Zhu,Tingting Fan,Yaohua Chen,Xingyue Huo,Yuping Li,Danyong Liu,Yin Cai,Chi Wai Cheung,Jing Tang,Jian Cui,Zhengyuan Xia

Journal of orthopaedic surgery (Hong Kong) 29:23094990211000168 PubMed33729061

2021

Gene expression profiling of primary fibrochondrocyte cultures in traumatic and degenerative meniscus lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Nuri Aydın,Bedri Karaismailoğlu,Merve Alaylıoğlu,Duygu Gezen-Ak,Büşra Şengül,Esin Candaş,Selma Yılmazer,Erdinç Dursun

Journal of medical virology 92:1266-1276 PubMed31944314

2020

Human cytomegalovirus infection in Iranian glioma patients correlates with aging and tumor aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Faezeh Maleki,Zohreh-Azita Sadigh,Farzin Sadeghi,Ahad Muhammadnejad,Mohammad Farahmand,Mahmoud Parvin,Reza Shirkoohi

Journal of chemical neuroanatomy 92:41-47 PubMed29860071

2018

Okadaic acid-induced tau hyperphosphorylation and the downregulation of Pin1 expression in primary cortical neurons.

Applications

Species

Unspecified reactive species

Derya Metin-Armağan,Duygu Gezen-Ak,Erdinç Dursun,İrem Lütfiye Atasoy,Arzu Karabay,Selma Yılmazer,Melek Öztürk

PloS one 12:e0188605 PubMed29176823

2017

Vitamin D receptor is present on the neuronal plasma membrane and is co-localized with amyloid precursor protein, ADAM10 or Nicastrin.

Applications

Unspecified application

Species

Unspecified reactive species

Erdinç Dursun,Duygu Gezen-Ak

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175703)

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 568) preadsorbed (AB175703)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (5)

Product promise