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AB150128

Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed

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(9 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

3 Images
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (AB150128)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (AB150128)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.The secondary antibody (orange) was ab150128 Alexa Fluor® 594 rabbit anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (AB150128)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (AB150128)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150128 Alexa Fluor® 594 rabbit anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (AB150128)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (AB150128)

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p><a href='/en-us/products/primary-antibodies/alexa-fluor-594-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab178000'>ab178000</a> - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control to complement this secondary antibody.</p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using Human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Bone research 12:27 PubMed38714649

2024

Multi-omics analysis of human tendon adhesion reveals that ACKR1-regulated macrophage migration is involved in regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xinshu Zhang,Yao Xiao,Bo Hu,Yanhao Li,Shaoyang Zhang,Jian Tian,Shuo Wang,Zaijin Tao,Xinqi Zeng,Ning-Ning Liu,Baojie Li,Shen Liu

Bioengineering (Basel, Switzerland) 11: PubMed38534518

2024

Regeneration Process of an Autologous Tissue-Engineered Trachea (aTET) in a Rat Patch Tracheoplasty Model.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Iwasaki,Koichi Deguchi,Ryosuke Iwai,Yasuhide Nakayama,Hiroomi Okuyama

Scientific reports 12:20279 PubMed36434016

2022

Successful tracheal regeneration using biofabricated autologous analogues without artificial supports.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Hiwatashi,Ryosuke Iwai,Yasuhide Nakayama,Takeshi Moriwaki,Hiroomi Okuyama

EJVES vascular forum 54:27-35 PubMed35128505

2022

Acute Phase Pilot Evaluation of Small Diameter Long iBTA Induced Vascular Graft "Biotube" in a Goat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ryuji Higashita,Yasuhide Nakayama,Yasuyuki Shiraishi,Ryosuke Iwai,Yusuke Inoue,Akihiro Yamada,Takeshi Terazawa,Tsutomu Tajikawa,Manami Miyazaki,Mamiko Ohara,Tadashi Umeno,Keitaro Okamoto,Tomonori Oie,Tomoyuki Yambe,Shinji Miyamoto

Scientific reports 9:11433 PubMed31391536

2019

Electrical stimulation shifts healing/scarring towards regeneration in a rat limb amputation model.

Applications

Unspecified application

Species

Unspecified reactive species

K M C Oliveira,J H Barker,E Berezikov,L Pindur,S Kynigopoulos,M Eischen-Loges,Z Han,M B Bhavsar,D Henrich,L Leppik

Retrovirology 16:9 PubMed30940160

2019

Analysis of herpes simplex type 1 gB, gD, and gH/gL on production of infectious HIV-1: HSV-1 gD restricts HIV-1 by exclusion of HIV-1 Env from maturing viral particles.

Applications

Unspecified application

Species

Unspecified reactive species

Sachith Polpitiya Arachchige,Wyatt Henke,Maria Kalamvoki,Edward B Stephens

European journal of vascular and endovascular surg 55:882-887 PubMed29661648

2018

One year Rat Study of iBTA-induced "Microbiotube" Microvascular Grafts With an Ultra-Small Diameter of 0.6 mm.

Applications

Unspecified application

Species

Unspecified reactive species

Daizo Ishii,Jun-Ichiro Enmi,Ryosuke Iwai,Kaoru Kurisu,Eisuke Tatsumi,Yasuhide Nakayama

Journal of virology 92: PubMed29093081

2018

Analysis of Select Herpes Simplex Virus 1 (HSV-1) Proteins for Restriction of Human Immunodeficiency Virus Type 1 (HIV-1): HSV-1 gM Protein Potently Restricts HIV-1 by Preventing Intracellular Transport and Processing of Env gp160.

Applications

Unspecified application

Species

Unspecified reactive species

Sachith Polpitiya Arachchige,Wyatt Henke,Ankita Pramanik,Maria Kalamvoki,Edward B Stephens

Redox biology 9:77-89 PubMed27423013

2016

A biphasic effect of TNF-α in regulation of the Keap1/Nrf2 pathway in cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gobinath Shanmugam,Madhusudhanan Narasimhan,Ramasamy Sakthivel,Rajesh Kumar R,Christopher Davidson,Sethu Palaniappan,William W Claycomb,John R Hoidal,Victor M Darley-Usmar,Namakkal Soorappan Rajasekaran
View all publications
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