AB150127

Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed

Name: Rabbit Anti Mouse (IgG) secondary antibody preadsorbed Alexa Fluor® 647
Brand: Abcam Limited
SKU: AB150127

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(5 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (AB150127)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (AB150127)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150127 Alexa Fluor® 647 rabbit anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (AB150127)

Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (AB150127)

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<a href='/en-us/products/unavailable/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-alexa-fluorreg-647-ab176103'>ab176103</a> - Mouse monoclonal IgG1 (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody." }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:66 PubMed39917005

2025

Deciphering the synergistic role of tyrosyl-tRNA synthetase 1 and yes-associated protein 1: Catalysts of malignant progression in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Zhou,Xin Zhang,Chongyang Zhang,Yu Wang,Jiaju Zhang,Yunxia Wang,Yongbo Sui

Cell reports. Medicine 5:101826 PubMed39536753

2024

IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Viviana Rubino,Michelle Hüppi,Sabine Höpner,Luigi Tortola,Noah Schnüriger,Hugo Legenne,Lea Taylor,Svenja Voggensperger,Irene Keller,Remy Bruggman,Marie-Noëlle Kronig,Ulrike Bacher,Manfred Kopf,Adrian F Ochsenbein,Carsten Riether

mSphere 9:e0014024 PubMed38564734

2024

MYST regulates DNA repair and forms a NuA4-like complex in the malaria parasite .

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Kalamuddin,Ahmad Rushdi Shakri,Chengqi Wang,Hui Min,Xiaolian Li,Liwang Cui,Jun Miao

Theranostics 9:5347-5358 PubMed31410219

2019

Evaluation of serum extracellular vesicles as noninvasive diagnostic markers of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Huayi Wang,Dengzhi Jiang,Wenzhe Li,Xiang Xiang,Jun Zhao,Bin Yu,Chen Wang,Zhaohui He,Ling Zhu,Yanlian Yang

Nature medicine 24:1113-1120 PubMed29942089

2018

Genetic deficiency of indoleamine 2,3-dioxygenase promotes gut microbiota-mediated metabolic health.

Applications

Unspecified application

Species

Unspecified reactive species

Ludivine Laurans,Nicolas Venteclef,Yacine Haddad,Mouna Chajadine,Fawaz Alzaid,Sarvenaz Metghalchi,Bruno Sovran,Raphael G P Denis,Julien Dairou,Marina Cardellini,Jose-Maria Moreno-Navarrete,Marjolene Straub,Sarah Jegou,Claire McQuitty,Thomas Viel,Bruno Esposito,Bertrand Tavitian,Jacques Callebert,Serge H Luquet,Massimo Federici,José Manuel Fernandez-Real,Remy Burcelin,Jean-Marie Launay,Alain Tedgui,Ziad Mallat,Harry Sokol,Soraya Taleb

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (AB150127)

Alexa Fluor® - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (AB150127)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (5)

Product promise