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Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 26 publications.

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Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

Alkaline Phosphatase

Applications

WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF

Clonality

Polyclonal

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Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/500 - 1/2000
Notes

-

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Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 26 publications.

Key facts

Description

Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

Alkaline Phosphatase

Applications

WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF

Clonality

Polyclonal

Pre-adsorbed

No

Concentration
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Properties

Form

Liquid

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.87% Sodium chloride, 0.79% Tris HCl, 0.01% Magnesium chloride, 0.001% Zinc chloride

Purification technique

Affinity purification

Purification notes

Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Diluted stock solutions should be used immediately and not be frozen, Do Not Freeze, Store undiluted

Notes

Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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