AB6729

Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)

Name: Rabbit Anti Mouse (IgG) secondary antibody AP (ab6729) | Abcam
Brand: Abcam Limited
SKU: AB6729
Price: 265 USD
Availability: InStock

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(28 Publications)

Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 28 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

IHC-Fr, ICC/IF, WB, IHC-P, Dot, ELISA, IM

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/2000", "notes":"" } } }

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Product details

Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 8 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Diluted stock solutions should be used immediately and not be frozen|Do Not Freeze|Store undiluted

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Proteomes 12: PubMed39585121

2024

The Non-Linear Profile of Aging: U-Shaped Expression of Myostatin, Follistatin and Intermediate Signals in a Longitudinal In Vitro Murine Cell Sarcopenia Model.

Applications

Unspecified application

Species

Unspecified reactive species

Janire Alonso-Puyo,Oihane Izagirre-Fernandez,Olatz Crende,Jesús Seco-Calvo,Ainhoa Fernandez-Atutxa,Diego Fernandez-Lazaro,Patricia Garcia-Gallastegi,Begoña Sanz

Translational oncology 49:102048 PubMed39186862

2024

Therapeutic potential of tLyp-1-EV-shCTCF in inhibiting liver cancer stem cell self-renewal and immune escape via SALL3 modulation in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhu,Zhihui Xie

Nature communications 14:3576 PubMed37328476

2023

Resource-aware construct design in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Di Blasi,Mara Pisani,Fabiana Tedeschi,Masue M Marbiah,Karen Polizzi,Simone Furini,Velia Siciliano,Francesca Ceroni

Transfusion 63:586-600 PubMed36752125

2023

Different-sized extracellular vesicles derived from stored red blood cells package diverse cargoes and cause distinct cellular effects.

Applications

Unspecified application

Species

Unspecified reactive species

Joo-Yeun Oh,Marisa B Marques,Xin Xu,Jindong Li,Kristopher R Genschmer,Edward Phillips,Melissa F Chimento,James Mobley,Amit Gaggar,Rakesh P Patel

Vaccines 10: PubMed35214698

2022

Vaccine Candidate Double Mutant Variants of Enterotoxigenic Heat-Stable Toxin.

Applications

Unspecified application

Species

Unspecified reactive species

Ephrem Debebe Zegeye,Yuleima Diaz,Pål Puntervoll

Development (Cambridge, England) 148: PubMed34344024

2021

Cyclic growth of dermal papilla and regeneration of follicular mesenchymal components during feather cycling.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Wu,Ting-Xin Jiang,Mingxing Lei,Chih-Kuan Chen,Shu-Man Hsieh Li,Randall B Widelitz,Cheng-Ming Chuong

Science advances 5:eaax1738 PubMed31555735

2019

BAP1 regulates epigenetic switch from pluripotency to differentiation in developmental lineages giving rise to BAP1-mutant cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffim N Kuznetsov,Tristan H Aguero,Dawn A Owens,Stefan Kurtenbach,Matthew G Field,Michael A Durante,Daniel A Rodriguez,Mary Lou King,J William Harbour

Vaccine 37:6405-6414 PubMed31515145

2019

Virus-like particle-display of the enterotoxigenic Escherichia coli heat-stable toxoid STh-A14T elicits neutralizing antibodies in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Morten L Govasli,Yuleima Diaz,Pål Puntervoll

Experimental and therapeutic medicine 17:3509-3517 PubMed30988731

2019

Edoxaban improves atrial fibrillation and thromboembolism through regulation of the Wnt-β-induced PI3K/ATK-activated protein C system.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefeng Shan,Zhiqiang Liu,Muhuyati Wulasihan,Songfeng Ma

Food & function 10:1364-1374 PubMed30735221

2019

Red wine extract preserves tight junctions in intestinal epithelial cells under inflammatory conditions: implications for intestinal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Nunes,Víctor Freitas,Leonor Almeida,João Laranjinha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (28)

Product promise