AB97043

Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)

Be the first to review this product! Submit a review

(3 Publications)

Suitable for ICC, WB, IHC-P, ELISA. Cited in 3 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

Unknown

Western blot - Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (AB97043)

All lanes:

Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8226-loading-control-ab8226'>ab8226</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/5000 dilution

Lanes 3 - 4:

Western blot - Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/20000 dilution

Lanes 5 - 6:

Western blot - Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97043) at 1/50000 dilution

Predicted band size: 36 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

WB, ELISA, ICC, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"(Chemiluminescent)." }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"(Primary)." } } }

style

left-column

Product details

Part of the AbExcel range.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA, 0.0095% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Ighg1

Additional targets

Ighg1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 28:101331 PubMed31568923

2019

Iodide modulates protein damage induced by the inflammation-associated heme enzyme myeloperoxidase.

Applications

Unspecified application

Species

Unspecified reactive species

Luke F Gamon,Simon Dieterich,Marta T Ignasiak,Verena Schrameyer,Michael J Davies

Oncology letters 13:893-897 PubMed28356975

2017

Potential regulation of glioma through the induction of apoptosis signaling via Egl-9 family hypoxia-inducible factor 3.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Mao,Chao You,Ding Lei,Heng Zhang

PLoS genetics 11:e1005093 PubMed25786024

2015

Male-biased aganglionic megacolon in the TashT mouse line due to perturbation of silencer elements in a large gene desert of chromosome 10.

Applications

IHC

Species

Mouse

Karl-F Bergeron,Tatiana Cardinal,Aboubacrine M Touré,Mélanie Béland,Diana L Raiwet,David W Silversides,Nicolas Pilon

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)

Western blot - Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (AB97043)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

Secondary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (3)

Product promise