AB7074

Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed

Name: Rabbit Anti Mouse (IgG) secondary antibody preadsorbed Biotin (ab7074) | Abcam
Brand: Abcam Limited
SKU: AB7074
Price: 265 USD
Availability: InStock

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(10 Publications)

Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 10 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images

ELISA - Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed (AB7074)

ELISA

Supplier Data

ELISA - Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed (AB7074)

ELISA results of ab7074 against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (blue line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin HRP and TMB-1000 substrate.

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Human

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

IHC-Fr, ELISA, ICC/IF, IHC-P, Dot, IM, WB

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/100000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 8:e09047 PubMed35299600

2022

Thymax, a gross thymic extract, exerts cell cycle arrest and apoptosis in Ehrlich ascites carcinoma in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Nariman K Badr El-Din,Azza I Othman,Maggie E Amer,Mamdooh Ghoneum

American journal of translational research 13:124-142 PubMed33527013

2021

TRF-20-M0NK5Y93 suppresses the metastasis of colon cancer cells by impairing the epithelial-to-mesenchymal transition through targeting Claudin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Na Luan,Yiquan Chen,Qingsong Li,Yali Mu,Qin Zhou,Xun Ye,Qun Deng,Limian Ling,Jian Wang,Jianwei Wang

Molecular medicine reports 22:2973-2980 PubMed32945406

2020

Different MAPK signal transduction pathways play different roles in the impairment of glucose‑stimulated insulin secretion in response to IL‑1β.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Ou,Zhongxiong Zheng,Ben Niu,Jian Su,Heng Su

Biochemical and biophysical research communication 529:740-746 PubMed32736701

2020

Megalin-mediated albumin endocytosis in cultured murine mesangial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mark A Bryniarski,Benjamin M Yee,Lee D Chaves,Corrine M Stahura,Rabi Yacoub,Marilyn E Morris

European cells & materials 40:21-37 PubMed32672345

2020

Modelling the catabolic environment of the moderately degenerated disc with a caprine ex vivo loaded disc culture system.

Applications

Unspecified application

Species

Unspecified reactive species

C Me Rustenburg,J W Snuggs,K S Emanuel,A Thorpe,C Sammon,C L Le Maitre,T H Smit

JOR spine 3:e1098 PubMed33015573

2020

Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie Binch,Joseph Snuggs,Christine L Le Maitre

Experimental and therapeutic medicine 18:4707-4717 PubMed31777559

2019

Effect of ATM on inflammatory response and autophagy in renal tubular epithelial cells in LPS-induced septic AKI.

Applications

Unspecified application

Species

Unspecified reactive species

Chenfei Zheng,Ying Zhou,Yueyue Huang,Bicheng Chen,Minmin Wu,Yue Xie,Xinxin Chen,Mei Sun,Yi Liu,Chaosheng Chen,Jingye Pan

JOR spine 2:e1049 PubMed31463463

2019

Aquaporin expression in the human and canine intervertebral disc during maturation and degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph W Snuggs,Rebecca E Day,Frances C Bach,Matthew T Conner,Rowena A D Bunning,Marianna A Tryfonidou,Christine L Le Maitre

Scientific reports 7:1367 PubMed28465522

2017

Gravimetric sensors operating at 1.1 GHz based on inclined c-axis ZnO grown on textured Al electrodes.

Applications

Unspecified application

Species

Unspecified reactive species

Girish Rughoobur,Mario DeMiguel-Ramos,José-Miguel Escolano,Enrique Iborra,Andrew John Flewitt

PloS one 10:e0130827 PubMed26121033

2015

Genetic Polymorphisms Associated with Hearing Threshold Shift in Subjects during First Encounter with Occupational Impulse Noise.

Applications

Unspecified application

Species

Unspecified reactive species

Yohann Grondin,Magda E Bortoni,Rosalinda Sepulveda,Elisa Ghelfi,Adam Bartos,Douglas Cotanche,Royce E Clifford,Rick A Rogers

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed

ELISA - Rabbit Anti-Mouse IgG H&L (Biotin) preadsorbed (AB7074)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (10)

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Product promise