Rabbit Anti-Mouse IgG H&L (HRP)

19 product images

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  Gao et al PLoS One. 2016 Apr 28;11(4):e0154419. doi: 10.1371/journal.pone.0154419. eCollection 2016. Fig2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.
  

  Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue (Panel B).

  Black arrows indicate double-positive cells.

  Paraffin-embedded, formalin-fixed human liver tissues were incubated with anti-IL-22 (ab18499) or α-SMA antibody (Anti-alpha smooth muscle Actin antibody [1A4] ab7817) overnight at 4°C followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 0.1 mol/L) for 20mins and blocking endogenous peroxidase activity with 0.3% H₂O₂. Revelation of primary antibody was carried out using horseradish peroxidase (HRP)-conjugated rabbit polyclonal anti-mouse (ab6728), secondary antibody followed by diamino-benzidine (DAB) and haematoxylin coloration. Double staining was performed with 3-amino-9-ethyl-carbazole (red color) for IL-22 and BCIP/ NBT (indigo color) for CD4.

  Positively stained cells were counted at high-power field (hpf, ×400) according to described protocols.

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  All lanes: Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade ab24834) at 1 µg/mL

  All lanes: HeLa histone prep at 1 µg/mL

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

  Predicted band size: 15 kDa

  Observed band size: 18 kDa

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Blocking and diluting buffer: 5% NFDM/TBST

  All lanes: Western blot - Anti-ERG antibody [EPR3864] - Chimeric (Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) ab214341) at 1/1000 dilution

  Lane 1: Rat heart lysates at 20 µg

  Lane 2: Rat brain lysates at 20 µg

  Lane 3: Mouse brain lysates at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

  Predicted band size: 54 kDa

  Observed band size: 55 kDa

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Blocking and diluting buffer: 5% NFDM/TBST.
  

  Exposure time: Lane 1: 30 seconds
  Lane 2: 70 seconds
  Lane 3: 50 seconds
  

  All lanes: Western blot - Anti-TXNIP antibody [EPR14774] – Mouse IgG2b (Chimeric) (Anti-TXNIP antibody [EPR14774] - Mouse IgG2b (Chimeric) ab210826) at 1/1000 dilution

  Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

  Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

  Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

  Predicted band size: 44 kDa

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  The membrane was blocked in 5% BSA in TBST for 1 hour, then incubated for 1 hour in primary antibody diluted in TBST.

  All lanes: Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484) at 1/5000 dilution

  Lane 1: Hela whole cell (Human)

  Lane 2: 3T3 cell (Mouse)

  Lane 3: Rat brain

  Lane 4: Xenopus laevis embryo

  Lane 5: Chicken Liver

  Lane 6: EBTr cell (Cow)

  Lane 7: CHO cell (Chinese hamster)

  Lane 8: Pig liver

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 36 kDa

  Observed band size: 40 kDa

  Exposure time: 10s

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Lane 1: Marker

  Lanes 2 - 5: Western blot - Anti-hnRNP K antibody [F45 P9 C7] (Anti-hnRNP K antibody [F45 P9 C7] ab23644) at 1 µg/mL

  Lane 2: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µg

  Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

  Lane 4: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg

  Lane 5: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 50 kDa

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  All lanes: Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade ab1220)

  Lane 1: Calf thymus histone lysate
  Lane 2: Calf thymus histone lysate with Histone H3 peptide - unmodified at 1 μg/ml
  Lane 3: Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (Human Histone H3 (mono methyl K9) peptide ab1771) at 1 μg/ml
  Lane 4: Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (Human Histone H3 (di methyl K9) peptide ab1772) at 1 μg/ml
  Lane 5: Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (Human Histone H3 (tri methyl K9) peptide ab1773) at 1 μg/ml
  Lane 6: Calf thymus histone lysate with Histone H3 (di methyl K4) peptide (Human Histone H3 (di methyl K4) peptide ab7768) at 1 μg/ml
  Lane 7: Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (Human Histone H3 (di methyl K27) peptide ab1781) at 1 μg/ml

  Secondary
  Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution
  developed using the ECL technique

  Performed under reducing conditions.

  Predicted band size: 17 kDa

  Exposure time: 1 minute

  All lanes: Western blot - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade ab1220)

  All lanes: Calf thymus histone lysate

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 15 kDa

  Exposure time: 1min

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Anti-beta Actin antibody [mAbcam 8224] - Loading Control ab8224 used on Xenopus embryo lysate (20 ug of lysate/lane).
  
  Secondary
  Rabbit polyclonal anti-mouse HRP was used as the secondary antibody (ab6728) and developed using the ECL technique.
  Performed under reducing conditions.
  
  Predicted band size : 42kD

  All lanes: Western blot - Anti-beta Actin antibody [mAbcam 8224] - Loading Control (Anti-beta Actin antibody [mAbcam 8224] - Loading Control ab8224)

  All lanes: Xenopus embryo lysate at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 41 kDa

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Blocking and diluting buffer: 5% NFDM/TBST

  All lanes: Western blot - Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) (Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) ab214341) at 1/5000 dilution

  All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

  Predicted band size: 54 kDa

  Observed band size: 55 kDa

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Note: although S. cerevisiae is not known to express beta Actin, Abcam believes that the band on lane 3 corresponds to Actin 1 (Swissprot ID: P60010, based on sequence similarity).
  

  Secondary antibody - rabbit anti-mouse HRP (ab6728)

  All lanes: Western blot - Anti-beta Actin antibody [mAbcam 8224] - Loading Control (Anti-beta Actin antibody [mAbcam 8224] - Loading Control ab8224) at 1 µg/mL

  Lane 1: Drosophila lysate at 20 µg

  Lane 2: S. pombe lysate at 20 µg

  Lane 3: S. cerevisiae lysate (Actin 1 - please see note) at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 41 kDa

  Observed band size: 42 kDa

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Lane 1: Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1/1000 dilution

  Lane 2: Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1/10000 dilution

  Lanes 3 - 4: Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1/500 dilution

  Lanes 1 - 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

  Lane 3: HEK293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

  Lane 4: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 41 kDa

  Exposure time: 10s

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

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  Image courtesy of an anonymous customer review.

  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Primary antibody incubated for 16 hours at 4°C.
  Blocking step performed using 5% milk for 1 hour at room temperature.

  All lanes: Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] ab9225) at 1/1000 dilution

  All lanes: whole cell lysate prepared from XL10Gold cells at 50 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

  Predicted band size: 8 kDa

  Observed band size: 6 kDa

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  All lanes: Western blot - Anti-FOXP3 antibody [mAbcam 450] (Anti-FOXP3 antibody [mAbcam 450] ab450) at 1 µg/mL

  All lanes: HEK293 cells overexpressing human FOXP3 at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 47 kDa

  Observed band size: 50 kDa

  Exposure time: 45s

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  This data was developed using the same antibody clone in a different formulation containing PBS and Azide (Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] ab14955).

  All lanes: Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] ab14955) at 0.5 µg/mL

  Lanes 1, 3, 5 and 7: Control HeLa Histone prep at 5 µg

  Lanes 2, 4, 6 and 8: Colecemid treated HeLa Histone prep at 5 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

  Predicted band size: 15 kDa

  Observed band size: 20 kDa

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  This image is courtesy of an anonymous customer review.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  ab81785 staining LC3A/B in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein block for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour. Anti-mouse HRP (ab6728) (1/200) was used as the secondary antibody.

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  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  All lanes: Western blot - Anti-PLK1 antibody [36-298] (Anti-PLK1 antibody [36-298] ab17057) at 1 µg/mL

  All lanes: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 68 kDa

  Observed band size: 66 kDa

  Exposure time: 2min

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  ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  ab6728 was used at dilution 1/1000 with the primary antibody Anti-ARF1 antibody [ARFS 3F1] ab11038 in ELISA. See the review on ab11038.

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  This image is courtesy of an anonymous customer review

  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

  Blocking Step: 5% BSA for 1 hour at 23°C
  

  This image was generated from the hybridoma version.

  All lanes: Western blot - Anti-MGMT antibody [MT3.1] (Anti-MGMT antibody [MT3.1] ab39253) at 1/500 dilution

  All lanes: Human OE33 cells - whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/1000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 22 kDa

  Observed band size: 21 kDa

  Exposure time: 30s