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Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF. Ideal for western blot. Cited in 605 publications.

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF

Clonality

Polyclonal

Reactivity data

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/1000
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/2000 - 1/10000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/1000 - 1/5000
Notes

-

Images

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Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF. Ideal for western blot. Cited in 605 publications.

Key facts

Description

Rabbit Anti-Mouse IgG H&L (HRP)

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF

Clonality

Polyclonal

Pre-adsorbed

No

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Purification technique

Affinity purification

Purification notes

HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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