Rabbit Anti-Mouse IgG H&L (HRP)

• IHC-P

AbReview20299****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

ab81785 staining LC3A/B in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein block for 5 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 1 hour. Anti-mouse HRP (ab6728) (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• ELISA

Supplier Data

ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

ELISA results of ab6728 against purified Mouse IgG protein. Each well was coated in duplicate with 10 µg of Mouse IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/185,000. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

• WB

AbReview20304****

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Blocking Step : 5% BSA for 1 hour at 23°C

This image was generated from the hybridoma version.

All lanes:

Western blot - Anti-MGMT antibody [MT3.1] (<a href='/en-us/products/primary-antibodies/mgmt-antibody-mt31-ab39253'>ab39253</a>) at 1/500 dilution

All lanes:

Human OE33 cells - whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 21 kDa

true

Exposure time: 30s

This image is courtesy of an anonymous Abreview

• WB

Project

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

All lanes:

Western blot - Anti-PLK1 antibody [36-298] (<a href='/en-us/products/primary-antibodies/plk1-antibody-36-298-ab17057'>ab17057</a>) at 1 µg/mL

All lanes:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

Predicted band size: 68 kDa

Observed band size: 66 kDa

true

Exposure time: 2min

• WB

Lab

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 30 seconds
Lane 2 : 70 seconds
Lane 3 : 50 seconds

All lanes:

Western blot - Anti-TXNIP antibody [EPR14774] - Mouse IgG2b (Chimeric) (<a href='/en-us/products/primary-antibodies/txnip-antibody-epr14774-mouse-igg2b-chimeric-ab210826'>ab210826</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

Predicted band size: 44 kDa

false

• WB

Project

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

This data was developed using the same antibody clone in a different formulation containing PBS and Azide (ab14955).

All lanes:

Western blot - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (<a href='/en-us/products/primary-antibodies/histone-h3-phospho-s10-antibody-mabcam-14955-ab14955'>ab14955</a>) at 0.5 µg/mL

Lane 1:

Control HeLa Histone prep at 5 µg

Lane 2:

Colecemid treated HeLa Histone prep at 5 µg

Lane 3:

Control HeLa Histone prep at 5 µg with Human Histone H3 (unmodified) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 4:

Colecemid treated HeLa Histone prep at 5 µg with Human Histone H3 (unmodified) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-unmodified-peptide-ab7228'>ab7228</a>)

Lane 5:

Control HeLa Histone prep at 5 µg with Human Histone H3 (phospho S28) peptide (<a href='/en-us/products/unavailable/human-histone-h3-phospho-s28-peptide-ab14793'>ab14793</a>)

Lane 6:

Colecemid treated HeLa Histone prep at 5 µg with Human Histone H3 (phospho S28) peptide (<a href='/en-us/products/unavailable/human-histone-h3-phospho-s28-peptide-ab14793'>ab14793</a>)

Lane 7:

Control HeLa Histone prep at 5 µg with Human Histone H3 (phospho S10) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-phospho-s10-peptide-ab11477'>ab11477</a>)

Lane 8:

Colecemid treated HeLa Histone prep at 5 µg with Human Histone H3 (phospho S10) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-phospho-s10-peptide-ab11477'>ab11477</a>)

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 20 kDa

false

• WB

Unknown

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

The membrane was blocked in 5% BSA in TBST for 1 hour, then incubated for 1 hour in primary antibody diluted in TBST.

All lanes:

Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (<a href='/en-us/products/primary-antibodies/gapdh-antibody-mabcam-9484-loading-control-ab9484'>ab9484</a>) at 1/5000 dilution

Lane 1:

Hela whole cell (Human)

Lane 2:

3T3 cell (Mouse)

Lane 3:

Rat brain

Lane 4:

Xenopus laevis embryo

Lane 5:

Chicken Liver

Lane 6:

EBTr cell (Cow)

Lane 7:

CHO cell (Chinese hamster)

Lane 8:

Pig liver

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

Predicted band size: 36 kDa

Observed band size: 40 kDa

false

Exposure time: 10s

• WB

Unknown

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) (<a href='/en-us/products/primary-antibodies/erg-antibody-epr3864-mouse-igg2b-chimeric-ab214341'>ab214341</a>) at 1/5000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

Predicted band size: 54 kDa

Observed band size: 55 kDa

false

• WB

Lab

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) (<a href='/en-us/products/primary-antibodies/erg-antibody-epr3864-mouse-igg2b-chimeric-ab214341'>ab214341</a>) at 1/1000 dilution

Lane 1:

Rat heart lysates at 20 µg

Lane 2:

Rat brain lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

Predicted band size: 54 kDa

Observed band size: 55 kDa

false

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.

Immunohistochemistry double staining identities IL-22 cells were produced by CD4 cells in liver tissue (Panel B).

Black arrows indicate double-positive cells.

Paraffin-embedded, formalin-fixed human liver tissues were incubated with anti-IL-22 (ab18499) or α-SMA antibody (ab7817) overnight at 4°C followed by heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 0.1 mol/L) for 20mins and blocking endogenous peroxidase activity with 0.3% H₂O₂. Revelation of primary antibody was carried out using horseradish peroxidase (HRP)-conjugated rabbit polyclonal anti-mouse (ab6728), secondary antibody followed by diamino-benzidine (DAB) and haematoxylin coloration. Double staining was performed with 3-amino-9-ethyl-carbazole (red color) for IL-22 and BCIP/ NBT (indigo color) for CD4.

Positively stained cells were counted at high-power field (hpf, ×400) according to described protocols.

Gao et al PLoS One. 2016 Apr 28;11(4):e0154419. doi: 10.1371/journal.pone.0154419. eCollection 2016. Fig2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ELISA

Unknown

ELISA - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

ab6728 was used at dilution 1/1000 with the primary antibody ab11038 in ELISA. See the review on ab11038.

• WB

Project

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Note : although S. cerevisiae is not known to express beta Actin, Abcam believes that the band on lane 3 corresponds to Actin 1 (Swissprot ID : P60010, based on sequence similarity).

Secondary antibody - rabbit anti-mouse HRP (ab6728)

All lanes:

Western blot - Anti-beta Actin antibody [mAbcam 8224] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8224-loading-control-ab8224'>ab8224</a>) at 1 µg/mL

Lane 1:

Drosophila lysate at 20 µg

Lane 2:

S. pombe lysate at 20 µg

Lane 3:

S. cerevisiae lysate (Actin 1 - please see note) at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

Predicted band size: 41 kDa

Observed band size: 42 kDa

true

• WB

AbReview20684****

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Primary antibody incubated for 16 hours at 4°C.
Blocking step performed using 5% milk for 1 hour at room temperature.

All lanes:

Western blot - Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (<a href='/en-us/products/primary-antibodies/m13-bacteriophage-coat-protein-g8p-antibody-rl-ph2-ab9225'>ab9225</a>) at 1/1000 dilution

All lanes:

whole cell lysate prepared from XL10Gold cells at 50 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

Predicted band size: 8 kDa

Observed band size: 6 kDa

false

Image courtesy of an anonymous Abreview.

• WB

Ap

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Lane 1:

Marker

Lanes 2 - 5:

Western blot - Anti-hnRNP K antibody [F45 P9 C7] (<a href='/en-us/products/primary-antibodies/hnrnp-k-antibody-f45-p9-c7-ab23644'>ab23644</a>) at 1 µg/mL

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

Lane 4:

Western blot - Jurkat whole cell lysate (<a href='/en-us/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 5:

Western blot - HEK-293 whole cell lysate (<a href='/en-us/products/cell-lysates/hek-293-whole-cell-lysate-ab7902'>ab7902</a>) at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution

Predicted band size: 50 kDa

false

• WB

Unknown

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

All lanes:

Western blot - Anti-FOXP3 antibody [mAbcam 450] - Mouse IgG3 (<a href='/en-us/products/primary-antibodies/foxp3-antibody-mabcam-450-mouse-igg3-ab450'>ab450</a>) at 1 µg/mL

Lane 1:

HEK293 cells overexpressing human FOXP3 at 20 µg

Lane 2:

HEK293 cells overexpressing human FOXP3 at 20 µg with Human FOXP3 peptide (<a href='/en-us/products/unavailable/human-foxp3-peptide-ab16809'>ab16809</a>)

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

false

Exposure time: 45s

• WB

Project

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

All lanes:

Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-antibody-mabcam-24834-nuclear-loading-control-and-chip-grade-ab24834'>ab24834</a>) at 1 µg/mL

All lanes:

HeLa histone prep at 1 µg/mL

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 18 kDa

false

• WB

Project

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

ab8224 used on Xenopus embryo lysate (20 ug of lysate/lane).

SecondaryRabbit polyclonal anti-mouse HRP was used as the secondary antibody (ab6728) and developed using the ECL technique.
Performed under reducing conditions.

Predicted band size : 42kD

All lanes:

Western blot - Anti-beta Actin antibody [mAbcam 8224] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8224-loading-control-ab8224'>ab8224</a>)

All lanes:

Xenopus embryo lysate at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

Predicted band size: 41 kDa

true

• WB

Project

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

All lanes : Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)

Lane 1 : Calf thymus histone lysate
Lane 2 : Calf thymus histone lysate with Histone H3 peptide - unmodified at 1 μg/ml
Lane 3 : Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 1 μg/ml
Lane 4 : Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 1 μg/ml
Lane 5 : Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (ab1773) at 1 μg/ml
Lane 6 : Calf thymus histone lysate with Histone H3 (di methyl K4) peptide (ab7768) at 1 μg/ml
Lane 7 : Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (ab1781) at 1 μg/ml

Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 17 kDa

Exposure time : 1 minute

All lanes:

Western blot - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-di-methyl-k9-antibody-mabcam-1220-chip-grade-ab1220'>ab1220</a>) at 1 µg/mL

Lane 1:

Calf thymus histone lysate

Lane 2:

Calf thymus histone lysate with Histone H3 peptide - unmodified

Lane 3:

Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-mono-methyl-k9-peptide-ab1771'>ab1771</a>)

Lane 4:

Calf thymus histone lysate with Human Histone H3 (di methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k9-peptide-ab1772'>ab1772</a>)

Lane 5:

Calf thymus histone lysate with Human Histone H3 (tri methyl K9) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-tri-methyl-k9-peptide-ab1773'>ab1773</a>)

Lane 6:

Calf thymus histone lysate with Human Histone H3 (di methyl K4) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k4-peptide-ab7768'>ab7768</a>)

Lane 7:

Calf thymus histone lysate with Human Histone H3 (di methyl K27) peptide (<a href='/en-us/products/proteins-peptides/human-histone-h3-di-methyl-k27-peptide-ab1781'>ab1781</a>)

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

Predicted band size: 15 kDa

true

Exposure time: 1min

• WB

Unknown

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Lane 1:

Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8226-loading-control-ab8226'>ab8226</a>) at 1/1000 dilution

Lane 2:

Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8226-loading-control-ab8226'>ab8226</a>) at 1/10000 dilution

Lanes 3 - 4:

Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8226-loading-control-ab8226'>ab8226</a>) at 1/500 dilution

Lanes 1 - 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

HEK293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Lane 4:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

Predicted band size: 41 kDa

false

Exposure time: 10s

• WB

Unknown

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

ab6728 was used at dilution 1/2000 with the primary antibody ab72009 in WB. See the review on ab72009.

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

false

• WB

Supplier Data

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB6728)

Western blot analysis of ab6728 at 1/1000 dilution. Secondary Antibody : Rabbit Anti-Mouse IgG HRP at 1/40000 dilution for 30 mins at RT.

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/1000 dilution

Lanes 2 - 4:

HeLa WC Lysate + GFP protein at 10 µg

Lane 5:

HeLa Whole Cell Lysate at 10 µg

Secondary

All lanes:

Rabbit Anti-Mouse IgG HRP at 1/40000 dilution

false