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Suitable for ICC, WB, IHC-P, ELISA. Ideal for western blot. Cited in 188 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046), expandable thumbnail
  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046), expandable thumbnail
  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046), expandable thumbnail
  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046), expandable thumbnail

Publications

Key facts

Host species
Rabbit
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
ICC, WB, IHC-P, ELISA
Clonality
Polyclonal

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Reactivity data

Application
ICC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/2000.00000 - 1/20000.00000
Notes

(Colorimetric). (Chemiluminescent 1/10000 - 1/50000).

Application
IHC-P
Reactivity
Reacts
Dilution info
1/200.00000 - 1/5000.00000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/10000.00000 - 1/100000.00000
Notes

(Primary)

Additional Targets

Alternative names

Recommended products

Suitable for ICC, WB, IHC-P, ELISA. Ideal for western blot. Cited in 188 publications.

Key facts

Description
Rabbit Anti-Mouse IgG H&L (HRP)
Host species
Rabbit
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
ICC, WB, IHC-P, ELISA
Clonality
Polyclonal
Pre-adsorbed
No
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: PBS, 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Part of the AbExcel range.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-beta Actin antibody [mAbcam 8224] - Loading Control ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97046, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046), expandable thumbnail

    Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

    All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1 μg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 μg per lane.

    Secondary
    Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/2000 dilution
    Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/10000 dilution
    Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/20000 dilution

    Developed using the
    Secondary

    All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046), expandable thumbnail

    Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

    All lanes: Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (Anti-Influenza A Virus Nucleoprotein antibody [C43] ab128193) at 1/1000 dilution

    All lanes: MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34

    Secondary

    All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

    Formalin-fixed, paraffin embedded human stomach tissue was stained for p53 using a mouse Anti-p53 antibody.

    ab97046 was used as secondary antibody at 1/100 dilution. DAB staining.

  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046), expandable thumbnail

    Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-CDK1 antibody [A17] ab18 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

    All lanes: Western blot - Anti-CDK1 antibody [A17] (Anti-CDK1 antibody [A17] ab18) at 5 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

    Lane 3: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 4: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 5: RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 20 µg

    Lane 6: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa

    Observed band size: 35 kDa

    Exposure time: 4min

  • Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046), expandable thumbnail

    Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

    Sample: Human Cell lysate - whole cell (human glioma cells), Loading amount: 20 µg, Specification: human glioma cells

    Blocking step: Milk as blocking agent for 1 hour, Concentration: 5 %, Temperature: 25°C

    Other product details: 1st Ab Dilution 1/5000 anti-actin Ab, Incubation time: 16 hours, Temperature: 4°C

    Secondary antibody: ab97046 used at 1:20,000

    Bands: Specific: 45 kDa

    Notes: primary Ab (anti-actin) was used as a loading control in 6 human glioma cell lines

    This image is courtesy of an anonymous Abreview

    Secondary

    All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Exposure time: 2s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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