Rabbit Anti-Mouse IgG H&L (HRP)

6 product images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-beta Actin antibody [mAbcam 8224] - Loading Control ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97046, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is taken from an identical assay without primary antibody.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1 μg/ml Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 μg per lane. Secondary Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/2000 dilution Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/10000 dilution Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/20000 dilution Developed using the

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

• 

  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  All lanes: Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (Anti-Influenza A Virus Nucleoprotein antibody [C43] ab128193) at 1/1000 dilution

  All lanes: MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution

  Predicted band size: 56 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  Formalin-fixed, paraffin embedded human stomach tissue was stained for p53 using a mouse Anti-p53 antibody.
  

  ab97046 was used as secondary antibody at 1/100 dilution. DAB staining.

• 

  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-CDK1 antibody [A17] ab18 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

  All lanes: Western blot - Anti-CDK1 antibody [A17] (Anti-CDK1 antibody [A17] ab18) at 5 µg/mL

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

  Lane 3: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 4: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 5: RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 20 µg

  Lane 6: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 34 kDa

  Observed band size: 35 kDa

  Exposure time: 4min

• 

  Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  Sample: Human Cell lysate - whole cell (human glioma cells), Loading amount: 20 µg, Specification: human glioma cells
  

  Blocking step: Milk as blocking agent for 1 hour, Concentration: 5 %, Temperature: 25°C

  Other product details: 1st Ab Dilution 1/5000 anti-actin Ab, Incubation time: 16 hours, Temperature: 4°C

  Secondary antibody: ab97046 used at 1:20,000

  Bands: Specific: 45 kDa

  Notes: primary Ab (anti-actin) was used as a loading control in 6 human glioma cell lines

  This image is courtesy of an anonymous Abreview

  Secondary

  All lanes: Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

  Developed using the ECL technique.

  Performed under non-reducing conditions.

  Exposure time: 2s