Rabbit Anti-Mouse IgG H&L (HRP)

• WB

Lab

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab18 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-CDK1 antibody [A17] (<a href='/en-us/products/primary-antibodies/cdk1-antibody-a17-ab18'>ab18</a>) at 5 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 20 µg

Lane 6:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

true

Exposure time: 4min

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046)

IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97046, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046)

Formalin-fixed, paraffin embedded human stomach tissue was stained for p53 using a mouse Anti-p53 antibody.

ab97046 was used as secondary antibody at 1/100 dilution. DAB staining.

• WB

Unknown

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046)

Sample : Human Cell lysate - whole cell (human glioma cells), Loading amount : 20 µg, Specification : human glioma cells

Blocking step : Milk as blocking agent for 1 hour, Concentration : 5 %, Temperature : 25°C

Other product details : 1st Ab Dilution 1/5000 anti-actin Ab, Incubation time : 16 hours, Temperature : 4°C

Secondary antibody : ab97046 used at 1 : 20,000

Bands : Specific : 45 kDa

Notes : primary Ab (anti-actin) was used as a loading control in 6 human glioma cell lines

This image is courtesy of an anonymous Abreview

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

true

Exposure time: 2s

• WB

Unknown

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046)

All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 μg/ml

Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 μg per lane.

Secondary
Lanes 1 - 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/2000 dilution
Lanes 3 - 4 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/10000 dilution
Lanes 5 - 6 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab97046) at 1/20000 dilution

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046)

true

Exposure time: 1min

• WB

Supplier Data

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (AB97046)

All lanes:

Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (<a href='/en-us/products/primary-antibodies/influenza-a-virus-nucleoprotein-antibody-c43-ab128193'>ab128193</a>) at 1/1000 dilution

All lanes:

MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34

Secondary

All lanes:

Western blot - Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution

Predicted band size: 56 kDa

false