AB7075

Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed

Name: Rabbit Anti-Mouse IgG (HRP) preadsorbed secondary antibody (ab7075) | Abcam
Brand: Abcam Limited
SKU: AB7075
Price: 265 USD
Availability: InStock

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(4 Publications)

Suitable for Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

ELISA - Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed (AB7075)

ELISA

Supplier Data

ELISA - Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed (AB7075)

ELISA results of ab7075 against purified Mouse IgG. (Red Line). Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

IHC-Fr, ICC, ELISA, IHC-P, WB, Dot, IM

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/2500", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/150000", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 10:592906 PubMed33569353

2021

Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic and Contributes to Opsonophagocytosis Resistance in Bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Bin Xu,Xiangkai Zhuge,Fang Tang,Xuhang Wang,Qianwen Gong,Rui Chen,Feng Xue,Jianjun Dai

Lipids in health and disease 19:144 PubMed32563265

2020

Ethanol-mediated upregulation of APOA1 gene expression in HepG2 cells is independent of de novo lipid biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Youcef Khodja,Mark E Samuels

Nature communications 9:3882 PubMed30250272

2018

Spatiotemporal dynamics of homologous recombination repair at single collapsed replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Donna R Whelan,Wei Ting C Lee,Yandong Yin,Dylan M Ofri,Keria Bermudez-Hernandez,Sarah Keegan,David Fenyo,Eli Rothenberg

Molecular pharmaceutics 10:1655-67 PubMed23485062

2013

Inhaled PLGA particles of prostaglandin E₁ ameliorate symptoms and progression of pulmonary hypertension at a reduced dosing frequency.

Applications

Species

Unspecified reactive species

Vivek Gupta,Nilesh Gupta,Imam H Shaik,Reza Mehvar,Eva Nozik-Grayck,Ivan F McMurtry,Masahiko Oka,Masanobu Komatsu,Fakhrul Ahsan

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed

ELISA - Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed (AB7075)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (4)

Product promise