Suitable for Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.
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Publications
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- Frontiers in cellular and infection microbiology 10:5929062021Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic and Contributes to Opsonophagocytosis Resistance in Bacteria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Sun,Bin Xu,Xiangkai Zhuge,Fang Tang,Xuhang Wang,Qianwen Gong,Rui Chen,Feng Xue,Jianjun DaiPubMed 33569353
- Lipids in health and disease 19:1442020Ethanol-mediated upregulation of APOA1 gene expression in HepG2 cells is independent of de novo lipid biosynthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoucef Khodja et. al.PubMed 32563265
- Nature communications 9:38822018Spatiotemporal dynamics of homologous recombination repair at single collapsed replication forks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonna R Whelan,Wei Ting C Lee,Yandong Yin,Dylan M Ofri,Keria Bermudez-Hernandez,Sarah Keegan,David Fenyo,Eli RothenbergPubMed 30250272
- Molecular pharmaceutics 10:1655-672013Inhaled PLGA particles of prostaglandin E₁ ameliorate symptoms and progression of pulmonary hypertension at a reduced dosing frequency.
Key facts
- Host species
- Rabbit
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Human
- Conjugation
- HRP
- Applications
- Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF
- Clonality
- Polyclonal
Immunogen
- Database link P01868
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info 1/500 - 1/2500 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/150000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/200 - 1/1000 | Notes - |
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.
Key facts
- Description
- Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed
- Host species
- Rabbit
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Human
- Conjugation
- HRP
- Applications
- Dot, IHC-Fr, IHC-P, IM, WB, ELISA, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Immunogen
- Database link P01868
- Specificity
No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
ELISA - Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed (ab7075)
ELISA results of ab7075 against purified Mouse IgG. (Red Line). Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.
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