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AB7070

Rabbit Anti-Mouse IgG H&L preadsorbed

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(2 Publications)

Suitable for WB, ELISA, IHC. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images
ELISA - Rabbit Anti-Mouse IgG H&L preadsorbed (AB7070)
  • ELISA

Supplier Data

ELISA - Rabbit Anti-Mouse IgG H&L preadsorbed (AB7070)

ELISA results of ab7070 against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG [red line] and Human IgG [blue line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Substrate.

Key facts

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Human

Pre-adsorbed

Yes

Applications

ELISA, WB, IHC

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/40000", "notes":"<p></p>" }, "IHC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology 593:1809-27 PubMed25605613

2015

The use of murine-derived fundic organoids in studies of gastric physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Michael A Schumacher,Eitaro Aihara,Rui Feng,Amy Engevik,Noah F Shroyer,Karen M Ottemann,Roger T Worrell,Marshall H Montrose,Ramesh A Shivdasani,Yana Zavros

PLoS pathogens 11:e1004663 PubMed25658601

2015

CD44 plays a functional role in Helicobacter pylori-induced epithelial cell proliferation.

Applications

Flow Cyt

Species

Unspecified reactive species

Nina Bertaux-Skeirik,Rui Feng,Michael A Schumacher,Jing Li,Maxime M Mahe,Amy C Engevik,Jose E Javier,Richard M Peek,Karen Ottemann,Veronique Orian-Rousseau,Gregory P Boivin,Michael A Helmrath,Yana Zavros
View all publications
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