Suitable for WB, ELISA, IHC. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Expected | Dilution info 1/2000 - 1/10000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/20000 - 1/40000 | Notes - |
Application IHC | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Suitable for WB, ELISA, IHC. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
No reaction was observed against Human Serum Proteins.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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ELISA results of ab7070 against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG [red line] and Human IgG [blue line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Substrate.
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