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Suitable for WB, ELISA, IHC. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

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Images

ELISA - Rabbit Anti-Mouse IgG H&L preadsorbed (AB7070), expandable thumbnail

Publications

Key facts

Host species
Rabbit
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Human
Applications
WB, ELISA, IHC
Clonality
Polyclonal

Immunogen

Reactivity data

Application
WB
Reactivity
Expected
Dilution info
1/2000 - 1/10000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/20000 - 1/40000
Notes

-

Application
IHC
Reactivity
Expected
Dilution info
1/1000 - 1/5000
Notes

-

Additional Targets

Alternative names

Recommended products

Suitable for WB, ELISA, IHC. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

Key facts

Description
Rabbit Anti-Mouse IgG H&L preadsorbed
Host species
Rabbit
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Human
Applications
WB, ELISA, IHC
Clonality
Polyclonal
Pre-adsorbed
Yes
Immunogen
Specificity

No reaction was observed against Human Serum Proteins.

Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

1 product image

  • ELISA - Rabbit Anti-Mouse IgG H&L preadsorbed (ab7070), expandable thumbnail

    ELISA - Rabbit Anti-Mouse IgG H&L preadsorbed (ab7070)

    ELISA results of ab7070 against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG [red line] and Human IgG [blue line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Substrate.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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