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AB6735

Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)

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Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF, WB.

View Alternative Names

Ig gamma-1 chain C region

1 Images
Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (AB6735)
  • WB

Unknown

Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (AB6735)

All lanes:

Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (<a href='/en-us/products/primary-antibodies/tubulin-antibody-yl1-2-loading-control-ab6160'>ab6160</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/2000 dilution

Lanes 3 - 4:

Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/10000 dilution

Lanes 5 - 6:

Western blot - Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/20000 dilution

Predicted band size: 36 kDa

true

Key facts

Host species

Rabbit

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alkaline Phosphatase

Excitation/Emission
Applications

IHC-P, Dot, IM, IHC-Fr, WB, ELISA, ICC/IF

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"<p></p>" } } }
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Product details

Part of the AbExcel range.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 8 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Ig gamma-1 chain C region
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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