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Suitable for WB, ICC, IHC-Fr, IHC-P, ELISA. Cited in 1 publication.

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Key facts

Host species

Rabbit

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

Beta-galactosidase

Applications

WB, ICC, IHC-Fr, IHC-P, ELISA

Clonality

Polyclonal

Immunogen

  • Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link P20759

Reactivity data

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ICC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-Fr

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/100 - 1/1000

Notes

-

Alternative names

Recommended products

Suitable for WB, ICC, IHC-Fr, IHC-P, ELISA. Cited in 1 publication.

Key facts

Description

Rabbit Anti-Rat IgG H&L (beta-galactosidase)

Host species

Rabbit

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

Beta-galactosidase

Applications

WB, ICC, IHC-Fr, IHC-P, ELISA

Clonality

Polyclonal

Pre-adsorbed

No

Immunogen
  • Native Full Length Protein corresponding to Rat Ig gamma-1 chain C region. Database link P20759
Specificity

Beta-galactosidase labelling is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems.

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Purification technique

Affinity purification Immunogen

Purification notes

ab136716 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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